Downregulation of miR-214 promotes dilated Cardiomyopathy Progression through PDE5A-Mediated cGMP regulation.

Autor: Yan J; Graduate School, Henan University of Chinese Medicine, Zhengzhou, 450046, China., Wang X; Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, 450002, China.; Laboratory of Cell Imaging, Henan University of Chinese Medicine, 6 Dongfeng Rd, Zhengzhou, 450002, Henan, China., Cao P; Graduate School, Henan University of Chinese Medicine, Zhengzhou, 450046, China., Li Q; Heart Center, First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, 450002, China., Wu H; Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, 450002, China. wuhong@hactcm.edu.cn.; Laboratory of Cell Imaging, Henan University of Chinese Medicine, 6 Dongfeng Rd, Zhengzhou, 450002, Henan, China. wuhong@hactcm.edu.cn.; Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou, 450002, China. wuhong@hactcm.edu.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 14; Vol. 14 (1), pp. 28070. Date of Electronic Publication: 2024 Nov 14.
DOI: 10.1038/s41598-024-78983-2
Abstrakt: Dilated cardiomyopathy (DCM) is a myocardial disorder resulting in a substantial decline in cardiac function and potentially leading to heart failure. This research combines bioinformatics analysis with empirical validation to explore the roles and mechanisms of miR-214 in DCM. Using the DEseq2 R package, a total of 125 differentially expressed circulating miRNAs (DE c-miRNAs) and 784 DE genes (DEGs) were identified. Cross-analysis between target genes of DE c-miRNAs and DEGs identified 124 common genes, and protein-protein interaction analysis of common genes identified 11 hub genes. Twelve DE c-miRNAs were further verified by quantifying their levels in the serum of DCM patients and healthy individuals. miR-214 levels were significantly decreased in serum from DCM patients, positively correlated with left ventricular ejection fraction and left ventricular fractional shortening. Further analysis showed that miR-214 directly targets and negatively regulates phosphodiesterase 5 A (PDE5A). Elevated PDE5A expression reduced cGMP levels; however, using sildenafil, a PDE5A inhibitor, reversed this effect, substantiating the regulatory mechanism of miR-214 on cGMP via PDE5A. These results provide new potential targets for the diagnosis and treatment of DCM.
Competing Interests: Declarations Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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