Protective effects of Juglans regia oil on lead acetate-induced reprotoxicity in rats: an antioxidant, histological and computational molecular study.

Autor: Badraoui R; Department of Biology, Faculty of Science, University of Hail, Hail, Saudi Arabia.; Section of Histology-Cytology, Faculty of Medicine of Tunis, University of Tunis El Manar, La Rabta-Tunis, Tunisia.; Laboratory of Histo-Embryology & Cytogenetics, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia., Gargouri M; Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Sfax, Sfax, Tunisia., Brahmi F; Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia., Ben-Nasr H; Department of Chemistry, Faculty of Science, University of Hail, Hail, Saudi Arabia., Bahrini I; Department of Biology, Faculty of Science, University of Hail, Hail, Saudi Arabia., Soussi A; Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Sfax, Sfax, Tunisia.
Jazyk: angličtina
Zdroj: Journal of the science of food and agriculture [J Sci Food Agric] 2024 Nov 14. Date of Electronic Publication: 2024 Nov 14.
DOI: 10.1002/jsfa.14024
Abstrakt: Background: Lead (Pb) is a metal that affects many body systems, primarily the male reproductive system. This study aimed to examine the phytochemical profiling and beneficial effects of Juglans regia oil (JRO) in alleviating lead-induced reprotoxicity-associated oxidative injury in rats. Adult male Wistar rats were randomly divided into four groups as follows: control group received no treatment, Pb group was exposed to 0.344 g kg -1 bw of Pb acetate, Pb + JRO group was co-treated with Pb plus walnut oil (0.9 g kg -1 bw) and JRO group received walnut oil only.
Results: Pb-treated rats showed significantly decreased gonado-somatic index, count and viability of sperm. Testosterone levels decreased in Pb-treated animals. Besides, Pb disrupted the oxidative/antioxidative status, the plasmatic lipase activity and testicular Pb content. Furthermore, Pb produced testis histopathological features, especially cell degeneration, atrophy and tubular disarrangement. Co-treatment with JRO was found to be effective for recovering the increased testicular oxidative damage-associated histological features in the Pb group. This was supported by the in silico modeling results.
Conclusion: The in silico analyses revealed that JRO compounds bound androgen receptor and RAC-alpha serine/threonine kinase with high affinities, which together with pharmacokinetic properties and molecular interactions satisfactorily support the beneficial in vivo findings in the Pb + JRO group of rats. © 2024 Society of Chemical Industry.
(© 2024 Society of Chemical Industry.)
Databáze: MEDLINE
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