Copper functionalized, pro-angiogenic, and skin regenerative scaffolds based on novel chitosan/APDEMS modified sepiolite-based formulation.

Autor: Gull A; School of Chemistry, University of the Punjab, Lahore, Pakistan., Hussain T; School of Chemistry, University of the Punjab, Lahore, Pakistan., Islam A; School of Chemistry, University of the Punjab, Lahore, Pakistan; Institute of Polymer and Textile Engineering, University of the Punjab, Lahore, Pakistan. Electronic address: atif.chem@pu.edu.pk., Ara C; Institute of Zoology, University of the Punjab, Lahore, Pakistan.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec; Vol. 283 (Pt 1), pp. 137538. Date of Electronic Publication: 2024 Nov 13.
DOI: 10.1016/j.ijbiomac.2024.137538
Abstrakt: Biomaterials-based scaffolds are extensively explored for their proangiogenic and tissue regenerative abilities. The present study aimed to develop wound healing scaffolds based on chitosan/aminopropyldiethoxymethylsilane (APDEMS) modified sepiolite, loaded with copper (0-0.25 g), characterized by FTIR, SEM, mechanical, TGA and analyzed biomedically. The FTIR and SEM confirmed the silane-induced cross-linking and incorporation of copper leading to better dispersion of individual components in the scaffolds. Based on other physicochemical observations, the best scaffold was CS/MS/Cu0.1 (99.5 % increased Young's modulus compared to chitosan, maximum swelling = 900 %, equilibrium time = 70 min); So, CS/MS/Cu0.1 and 0.25 were chosen for further analysis. The CAM assay showed significantly increased angiogenesis in CS/MS/Cu0.1 and 0.25 groups, lacking any developmental anomalies in chick embryos, at lower copper concentrations. The scaffolds' wound healing potential and in-vivo toxicity were assessed by wound excision and histopathology of various organs in mice, respectively. The rate of wound contraction in the CS/MS/Cu0.1 group was significantly (P < 0.05) greater than the control. The abovementioned results corroborated the histological and biochemical findings regarding more collagen deposition in regenerated skin sections and insignificant deviations in biochemical parameters of treated mice, respectively. The formulated biomaterials have proven promising materials for promoting angiogenesis in chick models and accelerating regeneration in mice skin.
Competing Interests: Declaration of competing interest The authors declared no competing interests.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: