Spatiotemporal dynamics during niche remodeling by super-colonizing microbiota in the mammalian gut.

Autor:	Urtecho G; Department of Systems Biology, Columbia University, New York, NY, USA., Moody T; Department of Systems Biology, Columbia University, New York, NY, USA; Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA., Huang Y; Department of Systems Biology, Columbia University, New York, NY, USA; Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA., Sheth RU; Department of Systems Biology, Columbia University, New York, NY, USA; Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA., Richardson M; Department of Systems Biology, Columbia University, New York, NY, USA; Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA., Descamps HC; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute for Diabetes, Obesity and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Kaufman A; Department of Systems Biology, Columbia University, New York, NY, USA., Lekan O; Department of Systems Biology, Columbia University, New York, NY, USA; Columbia College, Columbia University, New York, NY 10027, USA., Zhang Z; Department of Systems Biology, Columbia University, New York, NY, USA; Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA., Velez-Cortes F; Department of Systems Biology, Columbia University, New York, NY, USA; Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA., Qu Y; Department of Systems Biology, Columbia University, New York, NY, USA., Cohen L; Department of Systems Biology, Columbia University, New York, NY, USA., Ricaurte D; Department of Systems Biology, Columbia University, New York, NY, USA; Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA., Gibson TE; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA; Computer Science and AI Lab, Massachusetts Institute of Technology, Cambridge, MA, USA., Gerber GK; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; MIT-Harvard Health Sciences and Technology, Cambridge, MA, USA., Thaiss CA; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute for Diabetes, Obesity and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Wang HH; Department of Systems Biology, Columbia University, New York, NY, USA; Department of Pathology and Cell Biology, Columbia University, New York, NY, USA; Columbia University Digestive and Liver Disease Research Center, New York, NY 10032, USA. Electronic address: harris.wang@columbia.edu.
Jazyk:	angličtina
Zdroj:	Cell systems [Cell Syst] 2024 Nov 20; Vol. 15 (11), pp. 1002-1017.e4. Date of Electronic Publication: 2024 Nov 13.
DOI:	10.1016/j.cels.2024.10.007
Abstrakt:	While fecal microbiota transplantation (FMT) has been shown to be effective in reversing gut dysbiosis, we lack an understanding of the fundamental processes underlying microbial engraftment in the mammalian gut. Here, we explored a murine gut colonization model leveraging natural inter-individual variations in gut microbiomes to elucidate the spatiotemporal dynamics of FMT. We identified a natural "super-donor" consortium that robustly engrafts into diverse recipients and resists reciprocal colonization. Temporal profiling of the gut microbiome showed an ordered succession of rapid engraftment by early colonizers within 72 h, followed by a slower emergence of late colonizers over 15-30 days. Moreover, engraftment was localized to distinct compartments of the gastrointestinal tract in a species-specific manner. Spatial metagenomic characterization suggested engraftment was mediated by simultaneous transfer of spatially co-localizing species from the super-donor consortia. These results offer a mechanism of super-donor colonization by which nutritional niches are expanded in a spatiotemporally dependent manner. A record of this paper's transparent peer review process is included in the supplemental information. Competing Interests: Declaration of interests H.H.W. is a scientific advisor of SNIPR Biome, Kingdom Supercultures, Fitbiomics, VecX Biomedicines, and Genus PLC and a scientific cofounder of Aclid and Foli Bio, all of whom are not involved in the study. R.U.S. is a cofounder of Kingdom Supercultures. (Copyright © 2024.)
Databáze:	MEDLINE
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