Multilocus sequence typing of clinical Burkholderia pseudomallei isolates from Cambodia.

Autor: Gyamfi E; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Delvallez G; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Cheng S; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.; LMI Drug Resistance In Southeast Asia, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Meng S; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Oeurn K; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Sam C; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Kerleguer A; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Guillard B; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Bañuls AL; LMI Drug Resistance In Southeast Asia, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.; MIVEGEC, Montpellier University, CNRS, IRD, Montpellier, France., Hide M; Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.; LMI Drug Resistance In Southeast Asia, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.; MIVEGEC, Montpellier University, CNRS, IRD, Montpellier, France.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Nov 14; Vol. 18 (11), pp. e0012652. Date of Electronic Publication: 2024 Nov 14 (Print Publication: 2024).
DOI: 10.1371/journal.pntd.0012652
Abstrakt: Melioidosis is a neglected tropical disease caused by Burkholderia pseudomallei, endemic to Southeast Asia and Northern Australia. Despite its increasing global public health and clinical significance, the molecular epidemiology of melioidosis and genetic diversity of B. pseudomallei in Cambodia remains poorly understood. This study aims to elucidate the genetic diversity and antibiotic susceptibility profiles of B. pseudomallei isolates responsible for melioidosis in humans. For this purpose, 14 clinical isolates cryopreserved at the Medical Biology Laboratory at Institut Pasteur du Cambodge from 2016 to 2020 were subjected to antimicrobial susceptibility testing and Multilocus Sequence Typing (MLST). Phenotypic testing revealed that 92.86% (13/14) of the isolates were sensitive to all tested antibiotics, while one isolate exhibited resistance to trimethoprim-sulfamethoxazole. MLST analysis resolved our isolates into 14 unique Sequence Types (STs), including 10 previously documented in Southeast Asia. Notably, ST1858, ST2064, ST2065, and ST2066 were identified as novel STs, while ST54, ST99, ST211, and ST1359 were reported in Cambodia for the first time in this study. Comparing our MLST data with available sequences on PubMLST (n = 165), our study unveiled a high genetic diversity of B. pseudomallei in Cambodia. The identified STs were closely associated with isolates from other Southeast Asian countries, particularly Thailand, Vietnam, and Malaysia. In conclusion, this study provided insight into the genetic diversity among B. pseudomallei clinical isolates in Cambodia and their close genetic association with Southeast Asian isolates. To further our understanding, a One Health approach, incorporating human, environmental (mainly soil), and animal compartments, is essential to decipher the epidemiology of B. pseudomallei in Cambodia.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Gyamfi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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