Field Trials of an Autonomous eDNA Sampler in Lotic Waters.

Autor:	George SD; U.S. Geological Survey, New York Water Science Center, Troy, New York 12180, United States., Sepulveda AJ; U.S. Geological Survey, Northern Rocky Mountain Science Center, Bozeman, Montana 59715, United States., Hutchins PR; U.S. Geological Survey, Northern Rocky Mountain Science Center, Bozeman, Montana 59715, United States., Pilliod DS; U.S. Geological Survey, Forest and Rangeland Ecosystem Science Center, Boise, Idaho 83702, United States., Klymus KE; U.S. Geological Survey, Columbia Environmental Research Center, Columbia, Missouri 65201, United States., Thomas AC; Smith-Root, Vancouver, Washington 98686, United States., Augustine BC; U.S. Geological Survey, Eastern Ecological Science Center, Laurel, Maryland 20708, United States., Huddleston Adrianza CC; U.S. Geological Survey, New York Water Science Center, Troy, New York 12180, United States., Jones DN; U.S. Geological Survey, Northern Rocky Mountain Science Center, Bozeman, Montana 59715, United States., Williams JR; Turner Institute of Ecoagriculture, Natural Resources Program, Bozeman, Montana 59718, United States., Leinonen EG; Turner Institute of Ecoagriculture, Natural Resources Program, Bozeman, Montana 59718, United States.
Jazyk:	angličtina
Zdroj:	Environmental science & technology [Environ Sci Technol] 2024 Nov 14. Date of Electronic Publication: 2024 Nov 14.
DOI:	10.1021/acs.est.4c04970
Abstrakt:	Environmental DNA (eDNA) analysis has become a transformative technology, but sample collection methods lack standardization and sampling at effective frequencies requires considerable field effort. Autonomous eDNA samplers that can sample water at high frequencies offer potential solutions to these problems. We present results from four case studies using a prototype autonomous eDNA sampler as part of the U.S. Geological Survey's Rapid Environmental eDNA Assessment and Deployment Initiative & Network (READI-Net) project. These case studies involved short-term deployments of an eDNA autosampler (Smith-Root) across a range of riverine habitats with the objectives of (a) identifying what insights could be gained from high-frequency autosampling and (b) benchmarking these autosamples against manually collected samples. The high frequency autosampling revealed high temporal variability of eDNA concentrations and provided valuable insights about eDNA associations with environmental covariates, such as discharge and turbidity. Benchmarking assessments indicated autosamples had similar detection rates to manual samples and obtained similar or greater eDNA quantities. We did find minimal carryover contamination in autosampler field controls. We conclude that eDNA autosamplers have potential to improve freshwater biosurveillance by reducing logistical sampling barriers, standardizing collection methods, and clarifying the influence of environmental covariates on eDNA results.
Databáze:	MEDLINE
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