On the Non-Catalytic Role of Lytic Polysaccharide Monooxygenases in Boosting the Action of PETases on PET Polymers.

Autor: Corrêa TLR; Department of Chemistry, University of York, Heslington, York, YO10 5DD, United Kingdom., Román EKB; Molecular and Morphofunctional Biology Graduate Program, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil., Costa CAR; Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil., Wolf LD; Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil., Landers R; Institute of Physics Gleb Wataghin, University of Campinas (UNICAMP), Campinas, Brazil., Biely P; Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic., Murakami MT; Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil., Walton PH; Department of Chemistry, University of York, Heslington, York, YO10 5DD, United Kingdom.
Jazyk: angličtina
Zdroj: ChemSusChem [ChemSusChem] 2024 Nov 14, pp. e202401350. Date of Electronic Publication: 2024 Nov 14.
DOI: 10.1002/cssc.202401350
Abstrakt: Synthetic polymers are resistant to biological attack, resulting in their long-term accumulation in landfills and in natural aquatic and terrestrial habitats. Lytic polysaccharide monooxygenases (LPMOs) are enzymes which oxidatively cleave the polysaccharide chains in recalcitrant polysaccharides such as cellulose. It has been widely hypothesised that LPMOs could be used to aid in the enzymatic breakdown of synthetic polymers. Herein, through the use of biochemical assays, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) we show that LPMOs can bind to polyethylene terephthalate (PET), and - in doing so - the hydrophobic surface of PET becomes more hydrophilic such that product release is boosted by subsequent treatment with classical PETases. The boosting effect is however, only observed in reactions when the LPMO and the PETase are added sequentially rather than simultaneously to the PET. Moreover, the same boosting effect is also seen when a catalytically-inactive mutant of LPMO is used, showing that the principal means by which AA9 LPMOs boost the degradation of synthetic polymers is through their role as a "hydrophobin" rather than as an oxygenase. Indeed, in accord with this role of LPMOs, we further show that this effect can be extended to other ostensibly 'non-catalytic' proteins beyond LPMOs, such as bovine serum albumin and lactate dehydrogenase.
(© 2024 The Authors. ChemSusChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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