The circulatory levels and bone expression of MIR21, 34a, 155 and their target genes in a section of Egyptian Population.

Autor: El-Tahan RA; Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria, 21561, Egypt., Oriquat GA; Department of Medical Laboratory, Amman University, Faculty of Allied Medical Sciences, Amman, 19328, Jordan., Sorour I; Department of Neurosurgery, Faculty of Medicine, Alexandria University, Alexandria, 21131, Egypt., Salem SM; Department of Neurosurgery, Faculty of Medicine, Alexandria University, Alexandria, 21131, Egypt., Kamel MA; Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria, 21561, Egypt.; Research Projects Unit, Pharos University in Alexandria, Alexandria, 21648, Egypt., Michel TN; Department of Physiology, Medical Research Institute, Alexandria University, Alexandria, 21561, Egypt., Abu-Samra N; Department of Basic Sciences, Faculty of Physical Therapy, Pharos University in Alexandria, Alexandria, 21648, Egypt. nehal_chemistry2005@yahoo.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 13; Vol. 14 (1), pp. 27779. Date of Electronic Publication: 2024 Nov 13.
DOI: 10.1038/s41598-024-77643-9
Abstrakt: Bone tissue is constantly regenerated and repaired through a finely balanced process known as bone remodeling. Many miRNAs act as regulators of the signaling pathways involved in bone metabolic processes to maintain tissue homeostasis. This study aimed to assess the circulating levels of MIR21, MIR34a, and MIR155 in human serum and their bone expression, and the expression of bone turnover-related genes which can reflect the bone quality. This prospective study was conducted on 60 patients (30 males and 30 females) indicated for surgical interventions for neural decompression +/- fixation. Relative quantification of expression of MIR21, miR34a, and MIR155 and bone related genes was assayed using PCR. The serum levels of osteocalcin and Serum Bone Alkaline Phosphatase (sBAP) were assayed using a human ELISA kit. The main finding of the present work was the strong positive association between the circulating levels of only miR21 and MIR155 and their bone expression in the population under study and with bone markers and target genes Also, a positive association was found between both bone expression and circulating MIR21 levels with age and sBAP. These results suggest that the circulating levels of these microRNAs as early markers for the predication of bone quality.
Competing Interests: Declarations Competing interests The authors declare no competing interests. Ethics approval This study was performed in line with the Declaration of Helsinki, and approved by Ethics Committee of Medical Research Institute, Alexandria University, Egypt. (Approval serial number : E/C, S/N, R18/2020 in October 2020).” for studies involving humans. Consent to participate Informed consent was obtained from all individual participants included in the study. Patient consent for publication Written informed consent has been obtained from the patient to publish this paper .
(© 2024. The Author(s).)
Databáze: MEDLINE
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