History of cervical excisional treatment is associated with changes in the cervical microbiota in women with preterm prelabor rupture of membranes.

Autor:	Matulova J; Department of Non-medical Studies, Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic., Musilova I; Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.; Department of Obstetrics and Gynecology, Hospital Most, o.z., Krajska zdravotni, Most, Czech Republic., Kukla R; Institute of Clinical Microbiology, Charles University, Faculty of Medicine in Hradec Kralove, Czech Republic., Bolehovska R; Institute of Clinical Microbiology, Charles University, Faculty of Medicine in Hradec Kralove, Czech Republic., Balcarova K; Department of Non-medical Studies, Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic., Wiik J; Department of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.; Department of Obstetrics and Gynecology, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden., Sengpiel V; Department of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.; Department of Obstetrics and Gynecology, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden., Bostik P; Institute of Clinical Microbiology, Charles University, Faculty of Medicine in Hradec Kralove, Czech Republic., Jacobsson B; Department of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.; Department of Obstetrics and Gynecology, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden.; Department of Genetics and Bioinformatics, Domain of Health Data and Digitalization, Institute of Public Health, Oslo, Norway., Kacerovsky M; Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.; Department of Obstetrics and Gynecology, Hospital Most, o.z., Krajska zdravotni, Most, Czech Republic.
Jazyk:	angličtina
Zdroj:	The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians [J Matern Fetal Neonatal Med] 2024 Dec; Vol. 37 (1), pp. 2425761. Date of Electronic Publication: 2024 Nov 13.
DOI:	10.1080/14767058.2024.2425761
Abstrakt:	Background: This study aimed to determine the differences in the cervical load and prevalence of Lactobacillus crispatus DNA, Lactobacillus iners DNA, Gardnerella vaginalis DNA , Sneathia sanguinegens DNA, and Ureaplasma species DNA between pregnant women with preterm prelabor rupture of membranes (PPROM) with and without a history of cervical excisional treatment. We also assessed the changes in the cervical load and prevalence of L. crispatus DNA, L. iners DNA, G. vaginalis DNA, S. sanguinegens DNA, and U. spp DNA. according to the cone length. Methods: This retrospective study included 132 women with singleton pregnancies complicated by PPROM. For all women, information about the cervical loads of bacterial DNA corresponding to L. crispatus , L. iners , G. vaginalis , S. sanguinegens , and U. spp., which was assessed using PCR, was available. Results: Women with a history of cervical excisional treatment had a higher cervical load of L. iners DNA (4.4 × 10 6 copies DNA/mL vs. 3.5 × 10 5 copies DNA/mL, p = .04) and a higher load and prevalence of U . spp. DNA (1.1 × 10 5 copies DNA/mL vs. 9.6 × 10 4 copies DNA/mL, p = .03; 2.7% vs. 0.5%, p = .04) than those without a history of cervical excisional treatment. In the subset of women with a history of cervical excisional treatment, those with a cone length 18 mm and more had a lower relative abundance of L. crispatus DNA (6% vs. 89%, p = .02), a higher load and relative abundance of L. iners DNA (1.1 × 10 7 copies DNA/mL vs. 8.2 × 10 5 copies DNA/mL, p = .04; 91% vs. 35%, p = .04), and higher loads of G. vaginalis DNA (7.6 × 10 4 copies DNA/mL vs. 3.2 × 10 2 copies DNA/mL, p = .02) than those with cone length < 18 mm. Conclusions: A history of cervical excisional treatment was associated with alterations in the cervical microbiota composition in pregnant women with PPROM.
Databáze:	MEDLINE
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