The Onset of Steatosis Occurs as Early as Seven Days and Progresses to Non-Alcoholic Steatohepatitis in a Pediatric Pig Model of Non-Alcoholic Fatty Liver Disease.

Autor:	Yadav R; School of Animal Sciences, Virginia Tech, Blacksburg, VA 24061., Gerrard SD; School of Animal Sciences, Virginia Tech, Blacksburg, VA 24061., Lima MRM; School of Animal Sciences, Virginia Tech, Blacksburg, VA 24061., Southard TL; Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061., Sunny NE; Department of Animal and Avian Sciences, University of Maryland, MD 20742., El-Kadi SW; School of Animal Sciences, Virginia Tech, Blacksburg, VA 24061. Electronic address: elkadi@vt.edu.
Jazyk:	angličtina
Zdroj:	The Journal of nutrition [J Nutr] 2024 Nov 11. Date of Electronic Publication: 2024 Nov 11.
DOI:	10.1016/j.tjnut.2024.11.009
Abstrakt:	Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic and progressive condition that afflicts patients of all ages, including neonates. Previously, we reported that neonatal pigs fed formulas rich in medium-chain (MCFA) compared with those fed long-chain fatty acid (LCFA) for 21 d, developed panacinar steatosis with no changes in whole body obesity. Objectives: The objective of the present study was to examine the temporal onset and development of NAFLD in neonatal pigs in response to MCFA feeding. Methods: Neonatal pigs (n=18) were fed isocaloric MCFA or LCFA. Six pigs from each group were euthanized following 7, 14 or 21 d of feeding. Body composition was assessed before initiation and at the end of the feeding period using dual-energy X-ray absorptiometry. Liver fat content and liver morphological features were determined from photomicrographs and evaluated for NAFLD by a pathologist. Results: Lean mass and fat mass as a percentage of body weight were not different between formulas. However, liver weight (P = 0.001) and liver fat mass (P < 0.001) were greater for pigs in the MCFA compared with those in the LCFA group. Steatosis developed as early as 7 d in the MCFA compared with the LCFA fed pigs (P < 0.001). In addition, steatosis progressed in a portal to venous direction as MCFA feeding duration was increased (P = 0.02). Pigs diagnosed with NASH (P < 0.001) and greater non-alcoholic fatty liver disease scores were those in the MCFA group (P < 0.001). Conclusions: These results suggest that the onset and progression of NAFLD from steatosis to NASH occurs rapidly in response to MCFA feeding. Moreover, periportal steatosis is the initial feature in the development of NAFLD before its progression to NASH. The development of NAFLD in neonates seems to occur independently of whole-body adiposity. Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 American Society for Nutrition. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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