Toxicological effects and potential reproductive risk of microplastic-induced molecular changes in protamine-like proteins and their DNA binding.

Autor: Marinaro C; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Scarciello G; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Bianchi AR; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Berman B; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Chianese T; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Scudiero R; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Rosati L; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., De Maio A; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy., Lettieri G; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy. Electronic address: gennarole@outlook.com., Piscopo M; Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy. Electronic address: marina.piscopo@unina.it.
Jazyk: angličtina
Zdroj: Chemico-biological interactions [Chem Biol Interact] 2024 Nov 12; Vol. 405, pp. 111309. Date of Electronic Publication: 2024 Nov 12.
DOI: 10.1016/j.cbi.2024.111309
Abstrakt: Today, plastic pollution is a widespread problem in all ecosystems and has a particularly severe impact on marine ecosystems and external fertilisers such as the mussel Mytilus galloprovincialis. The present study aims to assess the toxicological reproductive health effects in this organism following exposure to two concentrations of polystyrene microplastics (PS-MPs) (0.5 and 1 μg/L), representative of conditions in the Mediterranean Sea. After exposure, the electrophoretic pattern of protamine-like (PL) proteins, the major basic protein component of Mytilus galloprovincialis sperm chromatin, was analysed. Compared to the unexposed condition, differences were observed by SDS-PAGE and an increased ability of PL to bind and protect DNA from oxidative damage was then measured, particularly for PL from mussels exposed to 1 μg/L PS-MPs. At this dose of PS-MPs, a reduced release of all PLs from the sperm nuclei was also observed, whereas the digestion by micrococcal nuclease did not show any significant differences between the exposed and the unexposed conditions. Finally, the possibility of poly(ADP)-ribosylation of the PLs was investigated. PL-II showed an increase in poly(ADP)-ribosylation after PS-MPs exposure, which may account for the difference in the ability of the PLs to bind DNA. In conclusion, while all the results might suggest a molecular mechanism of gametic plasticity occurring upon exposure of mussels to PS-MPs 1 μg/L, they also indicate that this dose of exposure could be extremely detrimental to the reproductive health of Mytilus galloprovincialis because it could prevent the release of basic nuclear proteins from the sperm DNA at fertilisation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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