Comprehensive effects of tea branch biochar on antibiotic resistance profiles and C/N/S cycling in the compost microbiota of animal manure.

Autor: Ma J; College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, China., Yang W; College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, China., Li S; Key Laboratory of Soil Pollution Bioremediation of Zhejiang Province, State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China., Yang Z; College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, China., Qiao C; Key Laboratory of Soil Pollution Bioremediation of Zhejiang Province, State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China., Liu D; Key Laboratory of Soil Pollution Bioremediation of Zhejiang Province, State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China. Electronic address: liudan@zafu.edu.cn., Wang M; Key Laboratory of Applied Biotechnology on Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China. Electronic address: meiwang@zafu.edu.cn.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Dec 20; Vol. 957, pp. 177457. Date of Electronic Publication: 2024 Nov 15.
DOI: 10.1016/j.scitotenv.2024.177457
Abstrakt: The comprehensive effects of exogenous additives on microbial-driven antibiotic resistance profiles and C/N/S conversion in animal manure composting remains uncertain. This study examined whether tea branch biochar could regulate the microflora involved in antibiotic resistance and C/N/S conversion during pig and chicken manure composting. Compared with the control treatment, biochar addition prolonged the high-temperature period (>55 °C) for 1-2 days and raised the maximum temperature in chicken manure composting. Moreover, biochar addition reduced the prevalence of antibiotic resistance genes (ARGs) in both pig and chicken manure composting by up to 30 %, targeting various types of ARGs such as peptide, phenicol, and diaminopyrimidines. Additionally, the compost microbiota exhibited the overlaps of C/N/S conversion functions. Luteimonas (Xanthomonadaceae) was identified as a dominant bacterium responsible for C/N/S conversion in both pig and chicken manure composting, while also acting as a potential ARG carrier. Thus, Luteimonas is crucial in shaping antibiotic resistance profiles and C/N/S cycling in animal manure composting, indicating its role as a keystone genus. These findings suggest that tea branch biochar can mitigate the spread of ARGs from animal manure, as well as enhance nutrient cycling and compost quality.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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