| Autor: |
Gest AMM; Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States., Sahan AZ; Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.; Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, California 92093, United States., Zhong Y; Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States., Lin W; Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States., Mehta S; Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States., Zhang J; Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, United States.; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States. |
| Abstrakt: |
Cellular function is controlled through intricate networks of signals, which lead to the myriad pathways governing cell fate. Fluorescent biosensors have enabled the study of these signaling pathways in living systems across temporal and spatial scales. Over the years there has been an explosion in the number of fluorescent biosensors, as they have become available for numerous targets, utilized across spectral space, and suited for various imaging techniques. To guide users through this extensive biosensor landscape, we discuss critical aspects of fluorescent proteins for consideration in biosensor development, smart tagging strategies, and the historical and recent biosensors of various types, grouped by target, and with a focus on the design and recent applications of these sensors in living systems. |
