Highly Tunable, Nanomaterial-Functionalized Structural Templating of Intracellular Protein Structures Within Biological Species.

Autor: Song DH; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Song CW; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Cho SH; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Kwon TY; Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Jung H; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Park KH; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Kim J; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Seo J; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Yoo J; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Kim M; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Lee GR; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Hwang J; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Lee HM; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Shin J; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Shin JH; Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Jung YS; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea., Chang JB; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea.; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea.; Bioimaging Data Curation Center, Seoul, 03760, South Korea.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Nov 13, pp. e2406492. Date of Electronic Publication: 2024 Nov 13.
DOI: 10.1002/advs.202406492
Abstrakt: Inside living organisms, proteins are self-assembled into diverse 3D structures optimized for specific functions. This structure-function relationship can be exploited to synthesize functional materials through biotemplating and depositing functional materials onto protein structures. However, conventional biotemplating faces limitations due to the predominantly intracellular existence of proteins and associated challenges in achieving tunability while preserving functionality. In this study, Conversion to Advanced Materials via labeled Biostructures (CamBio), an integrated biotemplating platform that involves labeling target protein structures with antibodies followed by the growth of functional materials, ensuring outstanding nanostructure tunability is proposed. Protein-derived plasmonic nanostructures created by CamBio can serve as precise quantitative tools for assessing target species is demonstrated. The assessment is achieved through highly tunable and efficient surface-enhanced Raman spectroscopy (SERS). CamBio enables the formation of dense nanogap hot spots among metal nanoparticles, templated by diverse fibrous proteins comprising densely repeated monomers. Furthermore, iterative antibody labeling strategies to adjust the antibody density surrounding targets, amplifying the number of nanogaps and consequently improving SERS performance are employed. Finally, cell-patterned substrates and whole meat sections as SERS substrates, confirming their easily accessible, cost-effective, scalable preparation capabilities and dimensional tunability are incorporated.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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