Phage Display Against 2D Metal-Organic Nanosheets as a New Route to Highly Selective Biomolecular Recognition Surfaces.

Autor: Wood AC; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Johnson EC; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Prasad RRR; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Sullivan MV; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Turner NW; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Armes SP; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Staniland SS; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK., Foster JA; Dainton Building, Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Nov 13, pp. e2406339. Date of Electronic Publication: 2024 Nov 13.
DOI: 10.1002/smll.202406339
Abstrakt: Peptides are important biomarkers for various diseases, however distinguishing specific amino-acid sequences using artificial receptors remains a major challenge in biomedical sensing. This study introduces a new approach for creating highly selective recognition surfaces using phage display biopanning against metal-organic nanosheets (MONs). Three MONs (ZIF-7, ZIF-7-NH 2, and Hf-BTB-NH 2 ) are added to a solution containing every possible combination of seven-residue peptides attached to bacteriophage hosts. The highest affinity peptides for each MON are isolated through successive bio-panning rounds. Comparison of the surface properties of the MONs and high-affinity peptides provide useful insights into the relative importance of electrostatic, hydrophobic, and co-ordination bonding interactions in each system, aiding the design of future MONs. Coating of the Hf-BTB-NH 2 MONs onto a quartz crystal microbalance (QCM) produced a five-fold higher signal for phage with the on-target peptide sequence compared to those with generic sequences. Surface plasmon resonance (SPR) studies produce a 4600-fold higher equilibrium dissociation constant (K D ) for on-target sequences and are comparable to those of antibodies (K D = 4 x 10 -10  m). It is anticipated that insights from the biopanning approach, combined with the highly tunable nature of MONs, will lead to a new generation of highly selective recognition surfaces for use in biomedical sensors.
(© 2024 The Author(s). Small published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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