Spatiotemporal Molecular Architecture of Lineage Allocation and Cellular Organization in Tooth Morphogenesis.

Autor: Jiang S; Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.; Institute of Medical Technology, Peking University Health Science Center, Beijing, 100191, P. R. China., Zhang Y; Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China., Zheng H; Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.; Institute of Medical Technology, Peking University Health Science Center, Beijing, 100191, P. R. China., Zhao K; Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.; Institute of Medical Technology, Peking University Health Science Center, Beijing, 100191, P. R. China., Yang Y; Department of Prosthodontics, The First Clinical Division, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China., Lai B; Biomedical Engineering Department, Institute of Advanced Clinical Medicine, Peking University, Beijing, 100191, P. R. China.; Department of Dermatology, Peking University First Hospital, Beijing, 100034, P. R. China., Deng X; Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.; Institute of Medical Technology, Peking University Health Science Center, Beijing, 100191, P. R. China., Wei Y; Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.; Institute of Medical Technology, Peking University Health Science Center, Beijing, 100191, P. R. China.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Dec; Vol. 11 (47), pp. e2403627. Date of Electronic Publication: 2024 Nov 13.
DOI: 10.1002/advs.202403627
Abstrakt: The remarkable evolution of teeth morphological complexity represents a giant leap for vertebrate. Despite its importance in life history, the understanding of spatiotemporal organization of teeth remains rudimentary. Herein, a high-resolution genome-wide molecular patterning of lineage allocation and cellular organization in tooth morphogenesis is described, constructed by integrating spatial transcriptome and single-cell RNA sequencing. Twelve spatial compartments and seventeen heterogeneous cell clusters linked to tooth morphogenic milestones are identified. Eighty-eight percent of total lineage species has already appeared in the initial tooth bud rather than the generally considered sequential emergence. A previously unrecognized sprouting-like patterning mode of the dental papilla is discovered, that the inner compartment can break through the outer shell compartment to build up the final papilla cusp. Meanwhile, the continuum differentiation hierarchies of enamel knots in time and space are revealed. Furthermore, the regulatory network directing tooth morphogenesis is established, whereby a series of mechanotransduction signals are spatiotemporally involved beyond the well-established classical odontogenesis signals. Finally, genes underlying tooth dysplasia are successfully tracked to highly specific time points and cell types. The results raise the idea that tooth morphogenesis is orchestrated by mechanical niches combined with biochemical signaling.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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