Synergistic induction of fertilization-independent embryogenesis in rice egg cells by paternal-genome-expressed transcription factors.

Autor: Ren H; Department of Plant Biology, University of California, Davis, CA, USA., Shankle K; Department of Plant Biology, University of California, Davis, CA, USA., Cho MJ; Innovative Genomics Institute, Berkeley, CA, USA., Tjahjadi M; Innovative Genomics Institute, Berkeley, CA, USA., Khanday I; Innovative Genomics Institute, Berkeley, CA, USA. khanday@ucdavis.edu.; Department of Plant Sciences, University of California, Davis, CA, USA. khanday@ucdavis.edu., Sundaresan V; Department of Plant Biology, University of California, Davis, CA, USA. sundar@ucdavis.edu.; Innovative Genomics Institute, Berkeley, CA, USA. sundar@ucdavis.edu.; Department of Plant Sciences, University of California, Davis, CA, USA. sundar@ucdavis.edu.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2024 Nov 12. Date of Electronic Publication: 2024 Nov 12.
DOI: 10.1038/s41477-024-01848-z
Abstrakt: In flowering plants, rapid activation of the zygotic genome occurs after fertilization 1-3 , but there is limited knowledge of the molecular pathways underlying embryo initiation 4 . In rice, a key role is played by the transcription factor BABY BOOM 1 (OsBBM1), initially expressed from the paternal genome 1 . Ectopic OsBBM1 expression in the egg cell can override the fertilization requirement, giving rise to parthenogenetic progeny 5 . Here we show that the WOX-family transcription factor DWARF TILLER1 (OsDWT1)/WUSCHEL-LIKE HOMEODOMAIN 9 (OsWOX9A) 6 , another gene paternally expressed in zygotes, is a strong enhancer of embryo initiation by OsBBM1. Co-expression of OsWOX9A and OsBBM1 in egg cells results in 86-91% parthenogenesis, representing 4- to 15-fold increases over OsBBM1 alone. These results suggest that embryo initiation is promoted by the synergistic action of paternal-genome-expressed transcription factors in the fertilized egg cell. These findings can be utilized for the efficient production of haploids, as well as clonal hybrid seeds in crop plants 7,8 .
Competing Interests: Competing interests The University of California, Davis, has filed a patent application on efficient induction of parthenogenesis in crop plants (PCT/US2023/034142) arising from this work. The authors declare no other competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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