Integrated multi-omics identifies pathways governing interspecies interaction between A. fumigatus and K. pneumoniae.

Autor: Bitencourt T; CCRI - St. Anna Children's Cancer Research Institute, Vienna, Austria.; Labdia - Labordiagnostik GmbH, Vienna, Austria.; Department of Medical Biochemistry, Campus Vienna Biocenter, Max Perutz Labs, Medical University of Vienna, Vienna, Austria., Nogueira F; CCRI - St. Anna Children's Cancer Research Institute, Vienna, Austria.; Labdia - Labordiagnostik GmbH, Vienna, Austria.; Department of Medical Biochemistry, Campus Vienna Biocenter, Max Perutz Labs, Medical University of Vienna, Vienna, Austria., Jenull S; Department of Medical Biochemistry, Campus Vienna Biocenter, Max Perutz Labs, Medical University of Vienna, Vienna, Austria.; Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Phan-Canh T; Department of Medical Biochemistry, Campus Vienna Biocenter, Max Perutz Labs, Medical University of Vienna, Vienna, Austria., Tscherner M; Department of Medical Biochemistry, Campus Vienna Biocenter, Max Perutz Labs, Medical University of Vienna, Vienna, Austria., Kuchler K; Department of Medical Biochemistry, Campus Vienna Biocenter, Max Perutz Labs, Medical University of Vienna, Vienna, Austria. kuchlerkarl1@gmail.com., Lion T; CCRI - St. Anna Children's Cancer Research Institute, Vienna, Austria. thomas.lion@ccri.at.; Labdia - Labordiagnostik GmbH, Vienna, Austria. thomas.lion@ccri.at.; Department of Pediatrics, Medical University of Vienna, Vienna, Austria. thomas.lion@ccri.at.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Nov 12; Vol. 7 (1), pp. 1496. Date of Electronic Publication: 2024 Nov 12.
DOI: 10.1038/s42003-024-07145-x
Abstrakt: Polymicrobial co- and superinfections involving bacterial and fungal pathogens pose serious challenges for diagnosis and therapy, and are associated with elevated morbidity and mortality. However, the metabolic dynamics of bacterial-fungal interactions (BFI) and the resulting impact on disease outcome remain largely unknown. The fungus Aspergillus fumigatus and the bacterium Klebsiella pneumoniae are clinically important pathogens sharing common niches in the human body, especially in the lower respiratory tract. We have exploited an integrated multi-omics approach to unravel the complex and multifaceted processes implicated in the interspecies communication involving these pathogens in mixed biofilms. In this setting, A. fumigatus responds to the bacterial challenge by rewiring its metabolism, attenuating the translational machineries, and by connecting secondary with primary metabolism, while K. pneumoniae maintains its central metabolism and translation activity. The flexibility in the metabolism of A. fumigatus and the ability to quickly adapt to the changing microenvironment mediated by the bacteria highlight new possibilities for studying the impact of cross-communication between competing interaction partners. The data underscore the complexity governing the dynamics underlying BFI, such as pronounced metabolic changes mounted in A. fumigatus interacting with K. pneumoniae. Our findings identify candidate biomarkers potentially exploitable for improved clinical management of BFI.
Competing Interests: Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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