Synergistic antibacterial effect of 405 nm blue light-emitting diodes (LEDs) and gelatin film for inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium on stainless steel and fresh fruit peel.

Autor: Koh N; Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea., Kim DK; Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: dkkim@snu.ac.kr.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2025 Jan 16; Vol. 427, pp. 110961. Date of Electronic Publication: 2024 Nov 07.
DOI: 10.1016/j.ijfoodmicro.2024.110961
Abstrakt: A combined antibacterial effect of 405 nm blue LEDs (BL) and gelatin film (G) was investigated on stainless steel (SUS) and fresh fruit peel for the inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium. On the SUS, the sum of the individual treatments of G for 20 min and BL at 20 J/cm 2 was <1 log reduction (log CFU/cm 2 ). In comparison, combination treatment of G and BL (G + BL) at 20 J/cm 2 exhibited 2.37 and 3.09 log reduction on E. coli O157:H7 and S. Typhimurium. The G + BL treatment only increased a propidium iodide (PI) uptake, indicating that cell membrane damage occurred. In the G + BL treatment, reactive oxygen species (ROS) scavenging assay confirmed that ROS involved in the bactericidal mechanism. On orange peel, the G + BL treatment at 40 J/cm 2 resulted in a 3.05 and 3.17 log reduction on E. coli O157:H7 and S. Typhimurium. In contrast, the individual treatment of G for 40 min led to reductions of 0.63 log CFU/cm 2 for E. coli O157:H7 and 0.50 log CFU/cm 2 for S. Typhimurium, while the BL treatment at 40 J/cm 2 achieved reductions of 0.78 and 0.69 log CFU/cm 2 , respectively. A synergistic bactericidal effect was similarly observed in the combined treatment groups for both apple and grapefruit peels. In a color and texture analysis, G did not affect hardness, toughness, and visual color of fruit.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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