Intracellular Mg 2+ concentrations are differentially regulated in the sperm head and mid-piece in acrosome reaction inducing conditions.

Autor: Sánchez-Cárdenas C; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, Mexico., De la Vega-Beltrán JL; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, Mexico., Weber WD; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA., Orta G; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, Mexico., Sánchez-Guevara Y; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, Mexico., Hernández-Cruz A; Departamento de Neuropatología Molecular y Laboratorio Nacional de Canalopatías, Instituto de Fisiología Celular UNAM, Ciudad Universitaria, Ciudad de México, Mexico., Darszon A; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, Mexico., Visconti PE; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA.
Jazyk: angličtina
Zdroj: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2024 Nov 15; Vol. 38 (21), pp. e70129.
DOI: 10.1096/fj.202401243R
Abstrakt: The sperm ability to fertilize involves the regulation of ATP levels. Because inside cells, ATP is complexed with Mg 2+ ions, changes in ATP levels result in changes in intracellular Mg 2+ concentration ([Mg 2+ ] i ), which can be followed using intracellular Mg 2+ sensors such as Mag-520. In this work, we tested conditions known to decrease sperm ATP such as starvation and capacitation. As expected, in these conditions [Mg 2+ ] i increased in all cell compartments. In contrast, when ATP increases, such as adding nutrients to starved sperm, [Mg 2+ ] i significantly decreases in all compartments. On the other hand, when the acrosome reaction was induced, either with progesterone or with ionomycin, [Mg 2+ ] i was differentially regulated in the head and mid-piece. While Mag-520 fluorescence increased in the sperm mid-piece, it decreased in the head. These changes were observed in capacitated as well as in starved sperm but not in sperm incubated in conditions that do not support capacitation. Changes in [Mg 2+ ] i were still observed when the sperm were incubated in high extracellular Mg 2+ suggesting that this decrease is not due to Mg 2+ efflux. Interestingly, the progesterone and ionomycin effects on [Mg 2+ ] i were abolished on sperm incubated in Ca 2+ -free media. Altogether, these results indicate that [Mg 2+ ] i is regulated in sperm during capacitation and acrosomal reaction, and suggest that these measurements can serve to evaluate ATP levels in real time.
(© 2024 Federation of American Societies for Experimental Biology.)
Databáze: MEDLINE
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