Bimodal Array-Based Fluorescence Sensor and Microfluidic Technology for Protein Fingerprinting and Clinical Diagnosis.

Autor:	Bosco MS; Unité de Technologies Chimiques et Biologiques pour la Santé - UTCBS, Faculté de Pharmacie de Paris, Université Paris Cité, CNRS UMR 8258, Inserm U1267, 75006 Paris, France., Naud-Martin D; Institut Curie, Université PSL, CNRS UMR9187, Inserm U1196, 91400 Orsay, France.; Université Paris-Saclay, CNRS UMR9187, Inserm U1196, 91400 Orsay, France., Gonzalez-Galindo C; Unité de Technologies Chimiques et Biologiques pour la Santé - UTCBS, Faculté de Pharmacie de Paris, Université Paris Cité, CNRS UMR 8258, Inserm U1267, 75006 Paris, France., Auvray M; Institut Curie, Université PSL, CNRS UMR9187, Inserm U1196, 91400 Orsay, France.; Université Paris-Saclay, CNRS UMR9187, Inserm U1196, 91400 Orsay, France., Araya-Farias M; Laboratoire Physics of Cells and Cancer (PCC), Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France., Gropplero G; Laboratoire Physics of Cells and Cancer (PCC), Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France., Rozenholc Y; BioSTM UR 7537, Faculté de Pharmacie de Paris, Université Paris Cité, 75006 Paris, France., Topcu Z; BioSTM UR 7537, Faculté de Pharmacie de Paris, Université Paris Cité, 75006 Paris, France., Gaucher JF; CiTCoM, Faculté de Pharmacie de Paris, Université Paris Cité, UMR CNRS 8038, 75006 Paris, France., Tsatsaris V; Department of Obstetric, Cochin Hospital, AP-HP, Université Paris Cité, FHU PREMA, 123 bd Port-Royal, 75014 Paris, France., Descroix S; Laboratoire Physics of Cells and Cancer (PCC), Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France., Mahuteau-Betzer F; Institut Curie, Université PSL, CNRS UMR9187, Inserm U1196, 91400 Orsay, France.; Université Paris-Saclay, CNRS UMR9187, Inserm U1196, 91400 Orsay, France., Gagey-Eilstein N; Unité de Technologies Chimiques et Biologiques pour la Santé - UTCBS, Faculté de Pharmacie de Paris, Université Paris Cité, CNRS UMR 8258, Inserm U1267, 75006 Paris, France.
Jazyk:	angličtina
Zdroj:	ACS applied bio materials [ACS Appl Bio Mater] 2024 Nov 12. Date of Electronic Publication: 2024 Nov 12.
DOI:	10.1021/acsabm.4c00938
Abstrakt:	Proteins play a crucial role in determining disease states in humans, making them prime targets for the development of diagnostic sensors. The developed sensor array is used to investigate global proteomic changes by fingerprinting multifactorial disease states in model urine simulating phenylketonuria and in serum from preeclamptic pregnant women. Here, we report a fluorescence-based chemical sensing array that exploits the host-guest interaction between cucurbit[7]uril (CB[7]) and fluorescent triphenylamine derivatives (TPA) to detect a range of proteins. Using linear discriminant analysis, we identify fluorescence fingerprints of 14 proteins with over 98% accuracy in buffer and human serum. The array is optimized on an automated droplet microfluidic-based platform, for high-throughput sensing with controlled composition and lower sample volumes. This sensor enables the discrimination of proteins in physiological buffer and human serum, with promising applications in disease diagnosis.
Databáze:	MEDLINE
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