E'jiao and Cubilose Formula Induced Antioxidant Activity and Improved Collagen Expression of Human Skin Fibroblasts During Oxidation Damage.

Autor: Tie H; Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, People's Republic of China.; School of Pharmacy, Tianjin Medical College, Tianjin, People's Republic of China.; Chinese Academy of Inspection & Quarantine Greater Bay Area, Zhongshan, Guangdong, People's Republic of China., Xie X; National Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Liaocheng, Shandong, China.; Dong-E E-Jiao Co. Ltd, Liaocheng, Shandong, China., Zhang Z; Tiangong University, Tianjin Medical College, Tianjin, People's Republic of China., Zhang J; National Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Liaocheng, Shandong, China.; Dong-E E-Jiao Co. Ltd, Liaocheng, Shandong, China., Xu L; Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, People's Republic of China.; School of Pharmacy, Tianjin Medical College, Tianjin, People's Republic of China., Ruan H; Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China., Wu T; Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China., Zhang H; Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China.
Jazyk: angličtina
Zdroj: Journal of cosmetic dermatology [J Cosmet Dermatol] 2024 Nov 12. Date of Electronic Publication: 2024 Nov 12.
DOI: 10.1111/jocd.16604
Abstrakt: Background: As traditional Chinese medicinal materials, E'jiao and cubilose are rich in various bioactive substances with good antioxidant, anti-inflammatory, and immune-regulating effects.
Aim: To obtain the optimal ratio of synergistic effect between E'jiao and cubilose.
Methods: The antioxidant capacity of E'jiao and cubilose digestive fluid was evaluated in vitro, as well as the intracellular oxidation balance between HSF cells and 3D whole-skin model induced by H 2 O 2 .
Results: E'jiao, cubilose, and their different ratios of composites had better scavenging ability against free radicals such as DPPH (2,2-Diphenyl-1-picrylhydrazyl). Using HSF cells induced by H 2 O 2 as an oxidative damage model, it was found that the combination of E'jiao and cubilose in a ratio of 2:3 significantly enhanced the activities of SOD, GSH-PX, and CAT enzymes compared to the separate treatments of E'jiao and cubilose, as well as other combination ratios. It effectively reduced the accumulation of reactive oxygen species caused by H 2 O 2 in HSF (Human Skin Fibroblast) cells and protected the integrity of the cells. Further analysis using flow cytometry and a 3D full-thickness skin model revealed that the combination ratio of 2:3 increased the proportion of H 2 O 2 -treated cells in the S%+G2% phase from 19.1% + 7.4%-22.1% + 28.8%, helping oxidatively damaged cells partially recover their proliferative capacity. It also promoted the expression of collagen I and collagen IV in the 3D full-thickness skin model, with improvement rates reaching 168.00% and 123.68%, respectively.
Conclusions: These findings indicate that the combination of E'jiao and cubilose in a ratio of 2:3 exhibits good synergistic effects, enhancing the ability of cells to resist oxidative damage, promoting cellular renewal and metabolism, and improving skin antiwrinkle capacity.
(© 2024 The Author(s). Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: