Virulence perspective genomic research unlocks the secrets of Rhizoctonia solani associated with banded sheath blight in Barnyard Millet ( Echinochloa frumentacea ).

Autor:	Patro TSSK; Agricultural Research Station, Acharya N. G. Ranga (ANGR) Agricultural University, Vizianagaram, Andhra Pradesh, India., Palanna KB; ICAR-All India Coordinated Research Project (ICAR-AICRP) on Small Millets, Project Coordinating (PC) Unit, University of Agricultural Sciences, Bengaluru, Karnataka, India., Jeevan B; Crop Protection Division, ICAR-National Rice Research Institute, Cuttack, Odisha, India., Tatineni P; Agricultural Research Station, Acharya N. G. Ranga (ANGR) Agricultural University, Vizianagaram, Andhra Pradesh, India., Poonacha TT; Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, Karnataka, India., Khan F; Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, Karnataka, India., Ramesh GV; Department of Plant Pathology, Punjab Agricultural University, Ludhiana, Punjab, India., Nayak AM; Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, Karnataka, India., Praveen B; Agricultural Research Station, Acharya N. G. Ranga (ANGR) Agricultural University, Vizianagaram, Andhra Pradesh, India., Divya M; Agricultural Research Station, Acharya N. G. Ranga (ANGR) Agricultural University, Vizianagaram, Andhra Pradesh, India., Anuradha N; Agricultural Research Station, Acharya N. G. Ranga (ANGR) Agricultural University, Vizianagaram, Andhra Pradesh, India., Rani YS; Agricultural Research Station, Acharya N. G. Ranga (ANGR) Agricultural University, Vizianagaram, Andhra Pradesh, India., Nagaraja TE; ICAR-All India Coordinated Research Project (ICAR-AICRP) on Small Millets, Project Coordinating (PC) Unit, University of Agricultural Sciences, Bengaluru, Karnataka, India., Madhusudhana R; ICAR- Indian Institute of Millets Research, Hyderabad, Telangana, India., Satyavathi CT; ICAR- Indian Institute of Millets Research, Hyderabad, Telangana, India., Prasanna SK; Department of Plant Biotechnology, University of Agricultural Sciences, Bengaluru, Karnataka, India.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Oct 28; Vol. 15, pp. 1457912. Date of Electronic Publication: 2024 Oct 28 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1457912
Abstrakt:	Introduction: Banded sheath blight (Bsb) disease, caused by Rhizoctonia solani , is an emerging problem in barnyard millet cultivation. One of the significant goals of pathogenomic research is to identify genes responsible for pathogenicity in the fungus. Methods: A virulence profiling-based approach was employed and six R. solani isolates were collected from various ecological zones of India. The morphological parameters and virulence of all of the six R. solani isolates were investigated. The most virulent strain was designated as RAP2 and its genome has been sequenced, assembled, and annotated. Results: The RAP2 genome is 43.63 megabases in size and comprises 10.95% repetitive DNA, within which 46% are retroelements, 8% are DNA transposons, and 46% are unidentified DNA. The Gene Ontology (GO) annotation of RAP2 proteins revealed that "phosphorylation", "membrane", and "ATP binding" have the highest gene enrichment in the "biological process", "cellular component" and "molecular function" domains, respectively. The genome comprises a majority of secretory proteins in the pectin lyase fold/virulence factor superfamily, which break down plant cell wall polymers to extract saccharides. The RAP2 genome is comparable to R. solani , which infects maize and rice, but it diverges further from soybean in terms of nucleotide-level genetic similarity. Orthologous clustering of RAP2 protein sequences with R. solani infecting maize, rice, and soybean yields 5606 proteins shared across all genomes. GO analysis of 25 proteins specific to the RAP2 genome found enrichment in the ethylene response, which can cause spore germination and infection in host plants. Discussion: Interestingly, a 28-bp deletion in the RAP2 strain's cutinase domain was discovered in the cutinase protein, which might be important in the infection process, perhaps rendering the enzyme inactive or allowing the pathogen to infect barnyard millet while avoiding host defense. This study sheds light on the genetic makeup of R. solani , allowing researchers to discover critical genes related with pathogenicity as well as potential targets for fungicide development. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Patro, Palanna, Jeevan, Tatineni, Poonacha, Khan, Ramesh, Nayak, Praveen, Divya, Anuradha, Rani, Nagaraja, Madhusudhana, Satyavathi and Prasanna.)
Databáze:	MEDLINE
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