Unraveling the impact of operational parameters and environmental conditions on the quality of viable bacterial aerosols.

Autor:	Thirugnanasampanthar M; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada., Tian L; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada., Rhem RG; Affiliate, Research Institute of St Joseph's Hospital and Firestone Institute for Respiratory Health, 50 Charlton Ave East, Hamilton, ON, L8N 4A6, Canada., Libera DD; Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada., Gomez M; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada., Jackson K; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.; Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, Canada., Fox-Robichaud AE; Department of Medicine, Faculty of Health Sciences, McMaster University, 1200 Main Street West, Hamilton, ON, L8N 3Z5, Canada.; Centre of Excellence in Protective Equipment and Materials, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada., Dolovich MB; Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, Canada.; Department of Medicine, Faculty of Health Sciences, McMaster University, 1200 Main Street West, Hamilton, ON, L8N 3Z5, Canada.; Centre of Excellence in Protective Equipment and Materials, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada., Hosseinidoust Z; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.; Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, Canada.; Centre of Excellence in Protective Equipment and Materials, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.; Michael DeGroote Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
Jazyk:	angličtina
Zdroj:	PNAS nexus [PNAS Nexus] 2024 Oct 30; Vol. 3 (11), pp. pgae473. Date of Electronic Publication: 2024 Oct 30 (Print Publication: 2024).
DOI:	10.1093/pnasnexus/pgae473
Abstrakt:	Viable pathogen-laden droplets of consistent quality are essential for reliably assessing the protection offered by facemasks against airborne infections. We identified a significant gap in guidance within standardized tests for evaluating the filtration efficiencies of facemask materials using viable bacteria-laden aerosol droplets. An aerosol platform, built according to the American Society for Testing and Materials standard F2101-19, was used to validate and standardize facemask filtration test procedures. We utilized this platform to investigate the impact of varying five operating parameters, namely suspension media composition, relative humidity, pathogen concentration, and atomizer airflow and feed flow rates, on the aerosol quality of viable bacteria-laden aerosols. We achieved consistent generation of 1,700 to 3,000 viable bacteria-laden droplets sized between 2.7 and 3.3 µm under the following optimized test conditions: 1.5% w/v peptone water concentration, ≥80% relative humidity at 24 ± 2 °C, 1 × 10 5 CFU/mL bacterial concentration, 1.5 L/min atomizer airflow rate, and 170 μL/min feed flow rate. We also explored the consequence of deviating from these optimized test parameters on viable bacteria-laden aerosol quality. These results highlight the importance of controlling these parameters when studying airborne transmission and control. (© The Author(s) 2024. Published by Oxford University Press on behalf of National Academy of Sciences.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu