Characterization and quantification of the phytochemical constituents and anti-inflammatory properties of Lindera aggregata .

Autor:	Wen SS; Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University Jinan 250012 China zhangnancy9@sdu.edu.cn.; NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Shandong Institute for Food and Drug Control Jinan 250101 China 13553158409@163.com., Liu YJ; Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University Jinan 250012 China zhangnancy9@sdu.edu.cn., Liu YL; Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University Shanghai 201306 China., Zhao Y; NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Shandong Institute for Food and Drug Control Jinan 250101 China 13553158409@163.com., Xu XX; Beijing Normal University Beijing 100875 China., Guo CC; NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Shandong Institute for Food and Drug Control Jinan 250101 China 13553158409@163.com., Niu C; NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Shandong Institute for Food and Drug Control Jinan 250101 China 13553158409@163.com., Wang WJ; NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Shandong Institute for Food and Drug Control Jinan 250101 China 13553158409@163.com., Xu YW; NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Shandong Institute for Food and Drug Control Jinan 250101 China 13553158409@163.com., Zhang N; Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University Jinan 250012 China zhangnancy9@sdu.edu.cn.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2024 Nov 11; Vol. 14 (48), pp. 36101-36114. Date of Electronic Publication: 2024 Nov 11 (Print Publication: 2024).
DOI:	10.1039/d4ra05643d
Abstrakt:	The dry roots of Lindera aggregata (Sims) Kosterm have a long-standing history in traditional Chinese medicine, renowned for their ability to regulate vital energy, relieve pain, warm the kidney, and dissipate cold. Recently, L. aggregata has been approved as a new food resource. To gain insights into the bioactive phytochemicals in L. aggregata , an ultrahigh-performance liquid chromatography coupled with high-resolution electrospray ionization quadrupole orbitrap spectrometry method was developed to investigate the chemical profiles of the ethanol extract of L. aggregata . This approach identified 80 compounds, predominantly alkaloids and sesquiterpenoids. Furthermore, 16 selected compounds were simultaneously quantified using the parallel reaction monitoring mode. The quantification method was validated and showed good linearity, sensitivity, and accuracy. The anti-inflammatory activities of the ethanol extract and selected compounds were assessed in vitro using lipopolysaccharide-stimulated RAW 264.7 macrophages. The results revealed that the ethanol extract of L. aggregata and norisoboldine, isolinderalactone, methyllinderone, and linderin B inhibited the production or expression of nitric oxide, inducible nitric oxide synthase (iNOS), tumor necrosis factor-α, and interleukin-6. Molecular docking of iNOS with isolinderalactone, methyllinderone, and linderin B showed that hydrogen bonds, π-π interactions, and hydrophobic interactions contributed to their iNOS inhibitory effects. The results offer insights that may be instrumental in enhancing the quality control for L. aggregata. Competing Interests: The authors declare no conflict of interest. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu