Nuclear localization sequence of MoHTR1, a Magnaporthe oryzae effector, for transcriptional reprogramming of immunity genes in rice.

Autor: Lim YJ; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Yoon YJ; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Lee H; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Choi G; Interdisciplinary Program in Agricultural Genomics, Seoul National University, Seoul, Republic of Korea., Kim S; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Ko J; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Kim JH; Department of Plant Medicine, Sunchon National University, Suncheon, Republic of Korea., Kim KT; Department of Plant Medicine, Sunchon National University, Suncheon, Republic of Korea.; Department of Agricultural Life Science, Sunchon National University, Suncheon, Republic of Korea., Lee YH; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. yonglee@snu.ac.kr.; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea. yonglee@snu.ac.kr.; Interdisciplinary Program in Agricultural Genomics, Seoul National University, Seoul, Republic of Korea. yonglee@snu.ac.kr.; Center for Fungal Genetic Resources, Seoul National University, Seoul, Republic of Korea. yonglee@snu.ac.kr.; Plant Immunity Research Center, Seoul National University, Seoul, Republic of Korea. yonglee@snu.ac.kr.; Center for Plant Microbiome Research, Seoul National University, Seoul, Republic of Korea. yonglee@snu.ac.kr.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Nov 11; Vol. 15 (1), pp. 9764. Date of Electronic Publication: 2024 Nov 11.
DOI: 10.1038/s41467-024-54272-4
Abstrakt: Plant pathogens secrete nuclear effectors into the host nuclei to modulate the host immune system. Although several nuclear effectors of fungal pathogens have been recently reported, the molecular mechanism of NLS-associated transport vehicles of nuclear effectors and the roles of NLS in transcriptional reprogramming of host immunity genes remain enigmatic. We previously reported the MoHTR1, a nuclear effector of the rice blast fungus, Magnaporthe oryzae. MoHTR1 is translocated to rice nuclei but not in fungal nuclei. Here, we identify the core NLS (RxKK) responsible for MoHTR1's nuclear localization. MoHTR1 is translocated in the host nucleus through interaction with rice importin α. MoHTR1 NLS empowers it to translocate the cytoplasmic effectors of M. oryzae into rice nuclei. Furthermore, other nuclear effector candidates of the blast pathogen and rice proteins which have RxKK also exhibit nuclear localization, highlighting the crucial role of RxKK in this process. We also unveil the importance of SUMOylation in the stability of MoHTR1 and translocation of MoHTR1 to host nuclei. Moreover, MoHTR1 NLS is essential for the pathogenicity of M. oryzae by reprogramming immunity-associated genes in the host. Our findings provide insights into the significance of plant-specific NLS on fungal nuclear effectors and its role in plant-pathogen interactions.
Competing Interests: Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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