Unveiling the impact of hypodermis on gene expression for advancing bioprinted full-thickness 3D skin models.

Autor: Avelino TM; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Harb SV; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Adamoski D; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Oliveira LCM; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Horinouchi CDS; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Azevedo RJ; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Azoubel RA; Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil., Thomaz VK; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil., Batista FAH; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil.; Molecular Research Laboratory in Cardiology, Dante Pazzanese Institute of Cardiology (IDPC), São Paulo, Brazil., d'Ávila MA; Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil., Granja PL; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, Porto, Portugal.; Instituto Nacional de Engenharia Biomédica (INEB), Universidade do Porto, Porto, Portugal., Figueira ACM; Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil. ana.figueira@lnbio.cnpem.br.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Nov 11; Vol. 7 (1), pp. 1437. Date of Electronic Publication: 2024 Nov 11.
DOI: 10.1038/s42003-024-07106-4
Abstrakt: 3D skin models have been explored as an alternative method to the use of animals in research and development. Usually, human skin equivalents comprise only epidermis or epidermis/dermis layers. Herein, we leverage 3D bioprinting technology to fabricate a full-thickness human skin equivalent with hypodermis (HSEH). The collagen hydrogel-based structure provides a mimetic environment for skin cells to adhere, proliferate and differentiate. The effective incorporation of the hypodermis layer is evidenced by scanning electron microscopy, immunofluorescence, and hematoxylin and eosin staining. The transcriptome results underscore the pivotal role of the hypodermis in orchestrating the genetic expression of a multitude of genes vital for skin functionality, including hydration, development and differentiation. Accordingly, we evidence the paramount significance of full-thickness human skin equivalents with hypodermis layer to provide an accurate in vitro platform for disease modeling and toxicology studies.
Competing Interests: Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje