Long-term effects of cycle time and volume exchange ratio on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production from food waste digestate by Haloferax mediterranei cultivated in sequencing batch reactors for 450 days.

Autor: Zhang X; Department of Biological System Engineering, Virginia Tech, Blacksburg, VA 24061, USA., An Z; Department of Biological System Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Wang J; Department of Biological System Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Lansing S; Department of Environmental Science and Technology, University of Maryland, Maryland, MD 20742, USA., Kumar Amradi N; Department of Environmental Science and Technology, University of Maryland, Maryland, MD 20742, USA., Sazzadul Haque M; Department of Biological System Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Wang ZW; Department of Biological System Engineering, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: wzw@vt.edu.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2025 Jan; Vol. 416, pp. 131771. Date of Electronic Publication: 2024 Nov 09.
DOI: 10.1016/j.biortech.2024.131771
Abstrakt: Food wastedigestatewas fed into a sequencing batch reactor (SBR) forHaloferax mediterranei(HM) to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). This SBR was operated uninterruptedly for 450 days to test its stability, during which the cycle time and volume exchange ratiowere varied to understand their impacts on the PHBV fermentation performance under ranged organic loading rates (OLR). Results showed that 1) PHBV productivity was proportional to OLR of food wastedigestate; 2) substrate and product inhibitions were two limiting factors constraining substrate utilization and PHBV yields; 3) PHBV titer was dependent on the hydraulic retention time of the SBR while a volume exchange ratio lower than 0.5 is unfavorable due to the product inhibitor accumulation. This study for the first time demonstrated that the long-term stability of food waste-fed PHBV production byHMand revealed that inhibition effects could be barriers in SBR limiting the full-scale application of the technology.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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