| Autor: |
Suzuki H; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan., Doura T; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan., Matsuba Y; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan., Matsuoka Y; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan., Araya T; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan., Asada H; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan., Iwata S; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.; RIKEN SPring-8 Center, Kobe, Hyogo 679-5148, Japan., Kiyonaka S; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.; Research Institute for Quantum and Chemical Innovation, Institutes of Innovation for Future Society, Nagoya University, Nagoya 464-8603, Japan. |
| Abstrakt: |
The use of photoresponsive ligands to optically control proteins of interest, known as photopharmacology, is a powerful technique for elucidating cellular function in living cells and animals with a high spatiotemporal resolution. The adenosine A 2A receptor (A 2A R) is a G protein-coupled receptor that is expressed in various tissues; its dysregulation is implicated in severe diseases such as insomnia and Parkinson's disease. A detailed elucidation of the physiological function of A 2A R is, therefore, highly desirable. In the present study, we developed two photoswitchable ligands, photoAd(blue) and photoAd(vio), that target A 2A R. Using photoAd(vio), we successfully demonstrated the selective activation of A 2A R in living cells by violet-light irradiation with high spatiotemporal resolution. |
