Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.

Autor:	Sivakova B; Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia., Jurcik J; Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia., Lukacova V; Medirex Group Academy n.o, Nitra, Slovakia., Lalakova LO; Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia., Selicky T; Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia., Cipakova I; Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia., Barath P; Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.; Medirex Group Academy n.o, Nitra, Slovakia., Cipak L; Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia. lubos.cipak@savba.sk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2862, pp. 297-307.
DOI:	10.1007/978-1-0716-4168-2_21
Abstrakt:	Protein phosphorylation is a dynamic, reversible posttranslational modification that plays an important role in the regulation of cell signaling. Recently, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex samples. In this chapter, we describe how to apply LFQ phosphoproteomics that is based on Fe-IMAC phosphopeptide enrichment followed by strong anion exchange (SAX) and porous graphitic carbon (PGC) fractionation strategies for identification and quantification of changes in the phosphoproteome in the fission yeast Schizosaccharomyces pombe. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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