CRISPR-Cas9 Genome Editing in Auxotrophic and Non-auxotrophic Fission Yeast Strains.
| Autor: | Hayashi A; Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, Japan. ahayashi@nibb.ac.jp., Nakayama JI; Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, Japan.; Basic Biology Program, Graduate Institute for Advanced Studies, Okazaki, Japan., Tanaka K; Department of Biosciences, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Japan. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2862, pp. 155-170. |
| DOI: | 10.1007/978-1-0716-4168-2_11 |
| Abstrakt: | The CRISPR/Cas system is a very powerful genome-editing tool that has been developed over the past decade to optimize genome editing for many organisms. Here, we describe a rapid genome-editing method for fission yeast using the CRISPR-Cas9 system. It allows rapid generation of desired auxotrophic and non-auxotrophic strains without perturbing the local genome content by avoiding the insertion of selection markers at target loci. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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