Serological and molecular detection of Toxoplasma gondii and Neospora caninum in ruminants from Somalia.

Autor: Kakimori MTA; Graduate Program On Animal Science, Universidade Estadual de Londrina, Londrina, Paraná, Brazil., Osman AM; Graduate Program On Veterinary Sciences, Universidade Federal Do Paraná, Curitiba, Paraná, Brazil. aamirmuse@gmail.com.; Somali One Health Centre, Abrar University, Muqdisho, Somalia. aamirmuse@gmail.com.; Department of Animal Health and Veterinary Services, Ministry of Livestock, Forestry, and Range, Mogadishu, Somalia. aamirmuse@gmail.com., Silva ACS; Graduate Program On Animal Science, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.; Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), The University of North Carolina at Charlotte, Charlotte, USA., Ibrahim AM; Somali One Health Centre, Abrar University, Muqdisho, Somalia.; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia., Shair MA; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia., Cavallieri AC; Graduate Program On Animal Science, Universidade Estadual de Londrina, Londrina, Paraná, Brazil., Barros LD; Department of Veterinary Medicine, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil., Garcia JL; Graduate Program On Animal Science, Universidade Estadual de Londrina, Londrina, Paraná, Brazil., Vieira TSWJ; Department of Chemistry, The University of North Carolina at Charlotte, Charlotte, USA., Hassan-Kadle AA; Somali One Health Centre, Abrar University, Muqdisho, Somalia. akadle@abrar.edu.so.; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia. akadle@abrar.edu.so., Vieira RFC; Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), The University of North Carolina at Charlotte, Charlotte, USA. rvieira@charlotte.edu.; Department of Epidemiology and Community Health, The University of North Carolina at Charlotte, Charlotte, USA. rvieira@charlotte.edu.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2024 Nov 11; Vol. 123 (11), pp. 376. Date of Electronic Publication: 2024 Nov 11.
DOI: 10.1007/s00436-024-08397-6
Abstrakt: Toxoplasma gondii and Neospora caninum infect a wide range of domestic and wild animals, including humans, in the case of T. gondii, and cause economic losses in livestock due to abortion and neonatal mortality. In Somalia, zoonotic diseases are concerning due to cultural practices and livestock's economic importance, but surveillance is limited. This study aimed to determine the seroprevalence and molecular prevalence of T. gondii and N. caninum in Somali sheep, goats, and cattle. A cross-sectional study was conducted between December 2018 and January 2020 in Benadir and Lower Shabelle regions of Somalia. Blood samples were collected from 128 cattle, 184 goats, and 46 sheep. Serum samples were tested for anti-T. gondii and anti-N. caninum antibodies using IFAT, and PCR was performed on extracted DNA to detect T. gondii and N. caninum DNA. Overall, 106/358 (29.6%) animals tested positive for anti-T. gondii antibodies, with the highest prevalence in sheep (62.5%), followed by goats (30.4%) and cattle (15.6%) (P < 0.001). For anti-N. caninum antibodies, 13/358 (3.6%) animals tested positive, with cattle showing the highest prevalence (6.2%), followed by goats and sheep (both 2.2%). Co-seropositivity for both antibodies was found in cattle and sheep. Molecular detection of T. gondii DNA revealed a prevalence of 9/358 (2.5%), primarily in sheep (15.2%) and cattle at 1.6% while all goat samples tested negative. No samples were positive for the N. caninum Nc5 gene. This study reveals T. gondii and N. caninum prevalence in Somali ruminants, highlighting the need for better surveillance and control.
Competing Interests: Declarations Ethics approval This study was approved by the ethical committee of Abrar University, Somalia (reference number for dromedary, sheep, and goats: AU/ARTC/EC/10/2018; for cattle: AU/ARTC/EC/06/2019). All livestock owners gave consent to sample their animals. Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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