| Autor: |
Egorova VV; ChemBioCluster, ITMO University, Saint Petersburg 191002, Russian Federation., Lavrenteva MP; ChemBioCluster, ITMO University, Saint Petersburg 191002, Russian Federation., Makhaeva LN; St. Petersburg Governor's Physics and Mathematics Lyceum N 30, Saint Petersburg 199004, Russian Federation., Petrova EA; Center for Chemical Engineering, ITMO University, Saint Petersburg 191002, Russian Federation., Ushakova AA; Center for Chemical Engineering, ITMO University, Saint Petersburg 191002, Russian Federation., Bozhokin MS; Russian Scientific Research Institute of Traumatology and Orthopedics Named After R.R. Vredena, Saint Petersburg 195427, Russian Federation.; Cytology Institute of Russian Academy of Sciences, Saint Petersburg 194064, Russian Federation., Krivoshapkina EF; ChemBioCluster, ITMO University, Saint Petersburg 191002, Russian Federation. |
| Abstrakt: |
One of the key strategies for tissue engineering is to design multifunctional bioinks that balance printability with cytocompatibility. Here, we describe fibrillar hydrogels produced by Schiff base formation between B-type gelatin and oxidized sodium alginate, followed by the incorporation of type I collagen, yielding a new gel (MyoColl). The resulting hydrogel exhibits a temperature- and mass-ratio-dependent sol-gel transition, showing variability of hydrogel properties depending on the component ratio. MyoColl composition provides a convenient platform for biofabrication in terms of shear thinning, yielding, Young's modulus, and shape accuracy. Metabolic activity tests and fluorescent microscopy of 2D hydrogel-based mouse C2C12 myoblast cell culture show significant cytocompatibility of the developed carriers. In addition, primary signs of cell mechanotransduction and myofilament formation of 3D printed MyoColl-based cell cultures were detected and described. Due to these promising results, the described hydrogel composition has shown itself as a convenient platform for muscle tissue engineering. |
