Metronidazole-laden silk fibroin methacrylated scaffolds for managing periapical lesions.

Autor: Silverberg A; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA., Cardoso LM; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), Araraquara School of Dentistry, 1680 Humaita Street, Araraquara, SP, 14801-903, Brazil., de Carvalho ABG; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), São Jose Dos Campos School of Dentistry, 777 Eng. Francisco Jose Longo Avenue, São Jose Dos Campos, SP, 12245-000, Brazil., Dos Reis-Prado AH; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.; Department of Restorative Dentistry, Minas Gerais Federal University (UFMG), School of Dentistry, 688 Prof. Moacir Gomes de Freitas Street, Belo Horizonte, MG, 31270-901, Brazil., Fenno JC; Department of Biologic and Materials Sciences & Prosthodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Ann Arbor, MI, 48109, USA., Dal-Fabbro R; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA., Bottino MC; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA. mbottino@umich.edu.; Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, MI, USA. mbottino@umich.edu.
Jazyk: angličtina
Zdroj: Odontology [Odontology] 2024 Nov 11. Date of Electronic Publication: 2024 Nov 11.
DOI: 10.1007/s10266-024-01023-y
Abstrakt: This study aimed to develop and characterize silk fibroin methacrylated/SilkMA electrospun scaffolds associated with metronidazole/MET to control infection in root-end resected periapical lesions while supporting bone regeneration. SilkMA-based formulations (10% w/v) incorporating MET (0-control; 5, 15, or 30% w/w) were electrospun into fibrous scaffolds and photocrosslinked. Scaffolds' morphology, chemical composition, swelling/degradation profiles, mechanical properties, cytocompatibility with alveolar bone-derived mesenchymal stem cells/aBMSCs and stem cells from apical papilla/SCAPs, anti-inflammatory potential, and antibacterial efficacy (direct contact assay against Aggregatibacter actinomycetemcomitans/Aa and Fusobacterium nucleatum/Fn; Aa biofilm model) were assessed. Statistical analysis was conducted using a significance level of 5%. Morphological analysis revealed that MET content influenced fiber diameters post-crosslinking, while the chemical composition analysis confirmed MET integration within the scaffolds. 30%MET-laden scaffolds demonstrated reduced swelling capacity compared to SilkMA/control scaffolds, while complete degradation was observed after 42 days for the formulated scaffolds. Mechanical testing indicated enhanced stiffness and tensile strength in 30%MET-laden scaffolds compared to SilkMA/control (p < 0.05). Cytocompatibility evaluations showed non-cytotoxic effects across all formulations for aBMSCs and SCAPs. Anti-inflammatory assays demonstrated decreased pro-inflammatory cytokine interleukin-6 synthesis by aBMSCs treated with SilkMA + MET30% and Escherichia coli LPS, comparable to negative control (p > 0.05). Antibacterial efficacy assays revealed significant inhibition of Aa and Fn, with 30%MET-laden scaffolds demonstrating biofilm inhibition against Aa (p < 0.05). These findings underscore the potential of SilkMA scaffolds laden with MET as a promising strategy for managing periapical lesions, offering enhanced structural support, antimicrobial properties, and biocompatibility crucial for effective tissue regeneration and infection control after endodontic surgery.
Competing Interests: Declarations Conflict of interest The authors declare that they have no conflicts of interest.
(© 2024. The Author(s), under exclusive licence to The Society of The Nippon Dental University.)
Databáze: MEDLINE
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