Impact of environmental factors on Apis dorsata (giant Asian honeybee) venom in Bandarawela, Sri Lanka.

Autor: Gunasekara P; Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka; Sri Lanka Institute of Biotechnology (SLIBTEC), SLIBTEC Phase I Building, Mahenawatte, Thalagala Road, Pitipana, Homagama, Sri Lanka. Electronic address: peshala.gunasekara@slibtec.gov.lk., Handunnetti SM; Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka., Premawansa S; Department of Zoology and Environment Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka., Dasanayake WMDK; Department of Immunology, Medical Research Institute, Colombo, Sri Lanka., Ratnayake IP; District Hospital, Bandarawela, Sri Lanka., Dias RKS; Department of Zoology and Environmental Management, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka., Premakumara GAS; Department of Basic and Social Sciences, Faculty of Nursing, University of Colombo, Sri Lanka., Seneviratne SL; Royal Free Hospital, United Kingdom; Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka., de Silva R; Department of Immunology, Medical Research Institute, Colombo, Sri Lanka.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Nov 08; Vol. 252, pp. 108163. Date of Electronic Publication: 2024 Nov 08.
DOI: 10.1016/j.toxicon.2024.108163
Abstrakt: Apis dorsata stings are amongst the most frequent insect-stings in Sri Lanka. A. dorsata venom consists of a mixture of components including PLA 2 , melittin, hyaluronidase and apamin. However, there are no studies done in Sri Lanka to evaluate the environmental influence in venom production. We, herein, tried to correlate the variations in those four venom components of A. dorsata with environmental factors (temperature, rainfall, wind speed and relative humidity). The venom components were analyzed using high-performance liquid chromatography (HPLC) and variations in environmental factors were gathered from the Department of Meteorology, Sri Lanka. Of the four components, PLA 2 (r = 0.718; p = 0.009 and r = 0.636; p = 0.026) and melittin r = 0.734; p = 0.007 and r = 0.582; p = 0.047) showed significant correlations with temperature and wind speed, respectively. Whilst relative humidity showed significant negative correlations with PLA 2 (r = - 0.634; p = 0.027) and melittin (r = -0.643; p = 0.024). There was no significant correlation of climatic parameters with hyaluronidase or apamin. The results indicated a variation in the composition of venom components with the month in which the venom was collected due to climatic influence and it should be considered when therapeutic grade venom preparations are produced.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr Rajiva de Silva reports financial support was provided by National Science Foundation, Sri Lanka. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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