Real-time simultaneous visualization of lactate and proton dynamics using a 6-μm-pitch CMOS multichemical image sensor.

Autor: Doi H; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan. Electronic address: doi.hideo.hj@tut.jp., Muraguchi H; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan., Horio T; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan., Choi YJ; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan., Takahashi K; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan; Institute for Research on Next-generation Semiconductor and Sensing Science (IRES(2)), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan., Noda T; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan; Institute for Research on Next-generation Semiconductor and Sensing Science (IRES(2)), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan., Sawada K; Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan; Institute for Research on Next-generation Semiconductor and Sensing Science (IRES(2)), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8122, Japan.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2025 Jan 15; Vol. 268, pp. 116898. Date of Electronic Publication: 2024 Oct 30.
DOI: 10.1016/j.bios.2024.116898
Abstrakt: Multi-analyte detection and imaging of extracellular chemical signaling molecules are crucial for understanding brain function and molecular pathology. In this work, we present a 6-μm-pitch, CMOS-based multichemical image sensor that enables the simultaneous visualization and spatiotemporal multimodal analysis of the lactate and proton (H + ) dynamics without any labeling. Using semiconductor lithography, gold electrode patterns functioning as lactate-sensing regions were formed on a potentiometric sensor array. Lactate is detected potentiometrically because of redox reactions using lactate oxidase and horseradish peroxidase. The resulting multichemical image sensor exhibited a pH sensitivity of 65 mV and a superior detection limit of 1 μM for lactate with a reasonable selectivity. Furthermore, diffusion images of lactate and H + distributions were obtained concurrently, allowing for simultaneous real-time imaging of the two chemicals with subcellular resolution. We believe that our novel imaging device can be successfully applied to extracellular microenvironments in tissue or cell samples as an effective bioimaging tool.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:To the best of our knowledge, the named authors have no conflict of interest, financial or otherwise.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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