In vitro antimicrobial and antioxidant activities of bioactive compounds extracted from Streptomyces africanus strain E2 isolated from Moroccan soil.

Autor: Rammali S; Laboratory of Agro-Alimentary and Health, Faculty of Sciences and Techniques, Hassan First University of Settat, B.P. 539, 26000, Settat, Morocco., Kamal FZ; Higher Institute of Nursing Professions and Health Technical (ISPITS), 40000, Marrakech, Morocco.; Laboratory of Physical Chemistry of Processes and Materials, Faculty of Sciences and Techniques, Hassan First University, 26000, Settat, Morocco., El Aalaoui M; Regional Center of Agronomic Research of Settat, Tertiary Road 1406, At 5 Km from Settat, 26400, Settat, Morocco., Bencharki B; Laboratory of Agro-Alimentary and Health, Faculty of Sciences and Techniques, Hassan First University of Settat, B.P. 539, 26000, Settat, Morocco., Burlui V; Department of Biomaterials, Faculty of Dental Medicine, Apollonia University, 700511, Iasi, Romania., Khattabi A; Laboratory of Agro-Alimentary and Health, Faculty of Sciences and Techniques, Hassan First University of Settat, B.P. 539, 26000, Settat, Morocco., Abderrahim A; Plant and Microbial Biotechnology Center, Moroccan Foundation for Advanced Science, Innovation and Research (MAScIR), Mohammed VI Polytechnic University, Ben Guerir, Morocco., Saad S; Laboratory of Biochemistry, Neurosciences, Natural Ressources and Environment, Faculty of Sciences and Techniques, Hassan First University of Settat, B.P. 539, 26000, Settat, Morocco., Romila L; Apollonia University, Păcurari Street 11, 700511, Iasi, Romania. laura.dartu@gmail.com., Novac B; Grigore T. Popa University of Medicine and Pharmacy, 16, Universitatii Street, 700115, Iasi, Romania., Aitlhaj-Mhand R; Virology Department, National Institute of Hygiene, Rabat, Morocco., Petroaie AD; Grigore T. Popa University of Medicine and Pharmacy, 16, Universitatii Street, 700115, Iasi, Romania., Ciobică A; Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 20th Carol I Avenue, 700506, Iasi, Romania.; Academy of Romanian Scientists, 3 Ilfov, 050044, Bucharest, Romania.; Clinical Department, Apollonia University, Păcurari Street 11, 700511, Iasi, Romania.; CENEMED Platform for Interdisciplinary Research, 'Grigore T. Popa' University of Medicine and Pharmacy of Iasi, 16th Universitatii Street, 700115, Iasi, Romania.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 09; Vol. 14 (1), pp. 27372. Date of Electronic Publication: 2024 Nov 09.
DOI: 10.1038/s41598-024-77729-4
Abstrakt: This study aimed to isolate Streptomyces sp. from Moroccan terrestrial ecosystems and identify bioactive compounds through GC-MS analysis. Antimicrobial activity was assessed against various pathogenic microorganisms including Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Candida albicans ATCC 60193, and multi-drug resistant strains comprising Listeria monocytogenes, Klebsiella pneumoniae 19K 929, Proteus sp. 19K1313, Klebsiella pneumoniae 20B1572, Proteus vulgaris 16C1737, and Klebsiella pneumoniae 20B1572. Based on the results of the gene sequencing of gene 16S rRNA and phylogenetic analysis, the E2 isolate belongs to the genus Streptomyces with the highest degree of resemblance (97.51%) to the Streptomyces africanus strain NBRC 101005 (NR_112600.1). The isolate exhibited broad-spectrum antibacterial activity, with maximum efficacy against Klebsiella pneumoniae 20B1572 indicated by an inhibition zone diameter of 22.5 ± 0.71mm and a minimum inhibitory concentration (MIC) of 0.0625 mg/mL. The in vitro antioxidant potential of E2 strain was determined through screening of its ethyl acetate extract against sets of antioxidant assays. The results were indicative of E2 strain displaying strong antioxidant activity against ABTS, DPPH free radicals, and FRAP. Furthermore, there was a high significant correlation (p < 0.0001) between the total phenolic and flavonoid content and antioxidant activities. The GC-MS analysis of the extract identified six volatile compounds, with Eugenol (96%) and Maltol (93%) being the most prominent. Additionally, the HPLC-UV/vis analysis revealed six phenolic compounds: gallic acid, chlorogenic acid, vanillic acid, trans-ferulic acid, ellagic acid, and cinnamic acid. Overall, the study highlights Streptomyces sp. strain E2 as a potential source of potent antimicrobial and antioxidant metabolites, offering promise in addressing antibiotic resistance and oxidative stress-related conditions.
(© 2024. The Author(s).)
Databáze: MEDLINE
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