Molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae in Gauteng South Africa.

Autor: Salvador-Oke KT; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa., Pitout JDD; Department of Pathology and Laboratory Medicine, Cummings School of Medicine, University of Calgary, Calgary, Canada.; Alberta Precision Laboratories, Calgary, Canada.; Tshwane Academic Division, Department of Medical Microbiology, National Health Laboratory Service, Pretoria, South Africa., Peirano G; Department of Pathology and Laboratory Medicine, Cummings School of Medicine, University of Calgary, Calgary, Canada.; Alberta Precision Laboratories, Calgary, Canada., Strydom KA; Tshwane Academic Division, Department of Medical Microbiology, National Health Laboratory Service, Pretoria, South Africa.; National Reference Laboratory, Ampath, Centurion, South Africa., Kingsburgh C; Tshwane Academic Division, Department of Medical Microbiology, National Health Laboratory Service, Pretoria, South Africa.; National Reference Laboratory, Ampath, Centurion, South Africa., Ehlers MM; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.; Tshwane Academic Division, Department of Medical Microbiology, National Health Laboratory Service, Pretoria, South Africa., Ismail A; Sequencing Core Facility, National Institute for Communicable Diseases a Division of the National Health Laboratory Service, Johannesburg, 2131, South Africa.; Department of Biochemistry and Microbiology, Faculty of Science, Engineering and Agriculture, University of Venda, Thohoyandou, 0950, South Africa.; Institute for Water and Wastewater Technology, Durban University of Technology, Durban, 4000, South Africa., Takawira FT; Research Circle Trust, Harare, Zimbabwe.; National Microbiology Reference Laboratory, Harare, Zimbabwe., Kock MM; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa. marleen.kock@up.ac.za.; Tshwane Academic Division, Department of Medical Microbiology, National Health Laboratory Service, Pretoria, South Africa. marleen.kock@up.ac.za.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 09; Vol. 14 (1), pp. 27337. Date of Electronic Publication: 2024 Nov 09.
DOI: 10.1038/s41598-024-70910-9
Abstrakt: Klebsiella pneumoniae multidrug-resistant (MDR) high-risk clones drive the spread of antimicrobial resistance (AMR) associated infections, resulting in limited therapeutic options. This study described the genomic characteristics of K. pneumoniae MDR high-risk clones in Gauteng, South Africa. Representative carbapenem-resistant [K. pneumoniae carbapenemase (KPC)-2, New-Delhi metallo-beta (β)-lactamase (NDM)-1, oxacillinase (OXA)-181, OXA-232, OXA-48, Verona integron-encoded metallo-β-lactamase (VIM)-1] K. pneumoniae isolates (n = 22) obtained from inpatient and outpatient's urine (n = 9) and inpatients rectal carriage (n = 13) were selected for short-read whole genome sequencing. Klebsiella pneumoniae population include sequence type (ST)-307 (n = 3), ST2497 (n = 5) and ST17 (n = 4). The ST17 strains were exclusively obtained from rectal screening. Ten isolates co-harboured carbapenemase genes including β-lactamase gene encoding KPC-2 + OXA-181, NDM-1 + OXA-48 and NDM-1 + OXA-181. One ST307 isolate (UP-KT-73CKP) co-harboured three carbapenemase genes (bla NDM-1  + bla OXA-48  + bla OXA-181 ), while all the ST2497 strains co-harboured (bla NDM-1  + bla OXA-232 ). Phenotypically, hypermucoviscosity was observed in a single ST307 isolate. The ST307 isolate UP-KT-151UKP harboured colibactin genotoxins. The following mobile genetic elements were detected: plasmids [incompatibility group (Inc)-FIB(K), IncX3], and bacteriophages [e.g. Klebsi_ST16_OXA48phi5.4_NC_049450, Klebsi_3LV2017_NC_047817(36)]. The study highlights the importance of local genomic surveillance systems to characterise K. pneumoniae MDR high-risk clones. This data will aid in designing infection and prevention measures for limiting the spread of carbapenemase-producing K. pneumoniae in Gauteng, South Africa.
(© 2024. The Author(s).)
Databáze: MEDLINE
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