Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin.

Autor: Iikawa H; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan., Nishina A; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan., Morita M; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan., Atsuta Y; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.; Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan., Hayashi Y; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.; Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan., Saito D; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.; Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Jazyk: angličtina
Zdroj: Development, growth & differentiation [Dev Growth Differ] 2024 Dec; Vol. 66 (9), pp. 452-461. Date of Electronic Publication: 2024 Nov 09.
DOI: 10.1111/dgd.12948
Abstrakt: Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally.
(© 2024 The Author(s). Development, Growth & Differentiation published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Developmental Biologists.)
Databáze: MEDLINE
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