| Autor: |
Akhtar Z; Scientific Direction Chemical and Physical Health Risks, Service of Medicines and Health Products, Sciensano, Rue Juliette Wytsmanstraat 14, B-1050 Brussels, Belgium.; Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, KU Leuven, Herestraat 49, O&N2, PB 923, B-3000 Leuven, Belgium., Canfyn M; Scientific Direction Chemical and Physical Health Risks, Service of Medicines and Health Products, Sciensano, Rue Juliette Wytsmanstraat 14, B-1050 Brussels, Belgium., Vanhee C; Scientific Direction Chemical and Physical Health Risks, Service of Medicines and Health Products, Sciensano, Rue Juliette Wytsmanstraat 14, B-1050 Brussels, Belgium., Delporte C; RD3-Pharmacognosy, Bioanalysis and Drug Discovery Unit, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), Bld Triomphe, Campus Plaine, CP 205/5, B-1050 Brussels, Belgium.; Analytical Platform of the Faculty of Pharmacy, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), Bld Triomphe, Campus Plaine, CP 205/5, B-1050 Brussels, Belgium., Adams E; Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, KU Leuven, Herestraat 49, O&N2, PB 923, B-3000 Leuven, Belgium., Deconinck E; Scientific Direction Chemical and Physical Health Risks, Service of Medicines and Health Products, Sciensano, Rue Juliette Wytsmanstraat 14, B-1050 Brussels, Belgium. |
| Abstrakt: |
The detection and quantification of additives in tobacco products are critical for ensuring consumer safety and compliance with regulatory standards. Traditional analytical techniques, like gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and others, although effective, suffer from drawbacks, including complex sample preparation, high costs, lengthy analysis times, and the requirement for skilled operators. This study addresses these challenges by evaluating the efficacy of mid-infrared (MIR) spectroscopy and near-IR (NIR) spectroscopy, coupled with multivariate analysis, as potential solutions for the detection and quantification of additives in tobacco products. So, a representative set of tobacco products was selected and spiked with the targeted additives, namely caffeine, menthol, glycerol, and cocoa. Multivariate analysis of MIR and NIR spectra consisted of principal component analysis (PCA), hierarchical clustering analysis (HCA), partial least squares-discriminant analysis (PLS-DA) and soft independent modeling of class analogy (SIMCA) to classify samples based on targeted additives. Based on the unsupervised techniques (PCA and HCA), a distinction could be made between spiked and non-spiked samples for all four targeted additives based on both MIR and NIR spectral data. During supervised analysis, SIMCA achieved 87-100% classification accuracy for the different additives and for both spectroscopic techniques. PLS-DA models showed classification rates of 80% to 100%, also demonstrating robust performance. Regression studies, using PLS, showed that it is possible to effectively estimate the concentration levels of the targeted molecules. The results also highlight the necessity of optimizing data pretreatment for accurate quantification of the target additives. Overall, NIR spectroscopy combined with SIMCA provided the most accurate and robust classification models for all target molecules, indicating that it is the most effective single technique for this type of analysis. MIR, on the other hand, showed the overall best performance for quantitative estimation. |
