Manipulation in root-associated microbiome via carbon nanosol for plant growth improvements.

Autor: Cheng L; Beijing Life Science Academy, Beijing, 102200, China.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Tao J; Beijing Life Science Academy, Beijing, 102200, China.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Lu P; Beijing Life Science Academy, Beijing, 102200, China.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Liang T; Key Laboratory of Ecological Environment and Tobacco Quality, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Li X; Henan Provincial Tobacco Company, Zhengzhou, 450001, China., Chang D; Henan Provincial Tobacco Company, Zhengzhou, 450001, China., Su H; Beijing Life Science Academy, Beijing, 102200, China.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., He W; Fujian Tobacco Industry Co., Ltd, Xiamen, 361001, China., Qu Z; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Li H; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Mu W; Beijing Life Science Academy, Beijing, 102200, China.; Key Laboratory of Ecological Environment and Tobacco Quality, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Zhang W; China National Tobacco Quality Supervision & Test Center, Zhengzhou, 450001, China., Liu N; China National Tobacco Quality Supervision & Test Center, Zhengzhou, 450001, China., Zhang J; Beijing Life Science Academy, Beijing, 102200, China.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China., Cao P; Beijing Life Science Academy, Beijing, 102200, China. peijiancao@163.com.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China. peijiancao@163.com.; School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China. peijiancao@163.com., Jin J; Beijing Life Science Academy, Beijing, 102200, China. jinjingjing1218@126.com.; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China. jinjingjing1218@126.com.
Jazyk: angličtina
Zdroj: Journal of nanobiotechnology [J Nanobiotechnology] 2024 Nov 09; Vol. 22 (1), pp. 685. Date of Electronic Publication: 2024 Nov 09.
DOI: 10.1186/s12951-024-02971-x
Abstrakt: Background: Modulating the microbiome with nanomaterials has been proposed to improve plant growth, and reduce reliance on external inputs. Carbon Nanosol (CNS) was attracted for its potential to improve plant productivity. However, the mechanism between CNS and rhizosphere microorganisms remained largely elusive.
Results: Here, we tried to systematically explore the effects of CNS (600 and 1200 mg/L by concentration) on tobacco growth, soil physical properties, and root-associated microbiome. The influence of CNS on soil physicochemical properties and plant growth was significant and dose-dependent, leading to a 28.82% increase in biomass accumulation by 600 mg/L CNS. Comparison between the CNS-treated and control plants revealed significant differences in microbiome composition, including 1148 distinct ASVs (923 bacteria and 225 fungi), microbiome interactions, and metabolic function of root-associated microbiomes. Fungal and bacterial communities had different response patterns for CNS treatment, with phased and dose-dependent effects, with the most significant changes in microbial community structure observed at 1200 mg/L after 10 days of treatment. Microbial networks of CNS-treated plants had more nodes and edges, higher connectivity, and more hub microorganisms than those of control plants. Compared with control, CNS significantly elevated abundances of various bacterial biomarkers (such as Sphingomonas and Burkholderia) and fungi biomarkers (including Penicillium, Myceliophthora, and Talaromyces), which were potential plant-beneficial organisms. Functional prediction based on metagenomic data demonstrated pathways related to nutrient cycling being greatly enriched under CNS treatment. Furthermore, 391 culturable bacteria and 44 culturable fungi were isolated from soil and root samples. Among them, six bacteria and two fungi strains enriched upon CNS treatment were validated to have plant growth promotion effect, and two fungi (Cladosporium spp. and Talaromyces spp.) played their roles by mediating volatile organic compounds (VOCs). To some extent, the driving and shaping of the microbiome by CNS contributed to its impact on plant growth and development.
Conclusion: Our results revealed the key role of root-associated microbiota in mediating the interaction between CNS and plants, thus providing valuable insights and strategies for harnessing CNS to enhance plant growth.
(© 2024. The Author(s).)
Databáze: MEDLINE
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