Interleukin-1β induces trained innate immunity in human hematopoietic progenitor cells in vitro.

Autor:	Flores-Gomez D; Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands., Hobo W; Department of Laboratory Medicine, Laboratory of Hematology, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands., van Ens D; Department of Laboratory Medicine, Laboratory of Hematology, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands., Kessler EL; Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands; Laboratory for Experimental Cardiology, Department of Cardiology, University Medical Center, Utrecht, 3584 CX Utrecht, the Netherlands., Novakovic B; Murdoch Children's Research Institute and Department of Pediatrics, University of Melbourne, Royal Children's Hospital, Parkville, VIC 3052, Australia., Schaap NPM; Department of Hematology, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands., Rijnen WHC; Department of Orthopedics, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands., Joosten LAB; Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands; Department of Medical Genetics, Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-Napoca 400347, Romania., Netea MG; Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands; Department of Immunology and Metabolism, Life and Medical Sciences Institute, University of Bonn, 53115 Bonn, Germany., Riksen NP; Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands. Electronic address: niels.riksen@radboudumc.nl., Bekkering S; Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, Gelderland, the Netherlands.
Jazyk:	angličtina
Zdroj:	Stem cell reports [Stem Cell Reports] 2024 Sep 25. Date of Electronic Publication: 2024 Sep 25.
DOI:	10.1016/j.stemcr.2024.09.004
Abstrakt:	Innate immune cells can develop a long-lasting hyperresponsive phenotype, termed trained immunity, mediated by epigenetic and metabolic reprogramming. In mice, exposure to Bacille Calmette-Guérin (BCG), β-glucan, or Western diet induces trained immunity by reprogramming hematopoietic progenitor cells (HPCs), through interleukin-1β (IL-1β) signaling in the bone marrow (BM). We investigated whether IL-1β induces trained immunity in primary human BM-derived HPCs in vitro. We exposed human BM-derived HPCs to IL-1β for 4 h. HPCs were expanded and differentiated into monocytes followed by functional and transcriptomic characterization. IL-1β-exposed HPCs showed higher granulocyte-macrophage colony-forming units. The monocyte offspring produced more tumor necrosis factor (TNF) and IL-1β after restimulation with lipopolysaccharide (LPS) and Pam3Cys and is metabolically more active. Transcriptomic analysis showed upregulation of key atherogenic and inflammatory pathways. In conclusion, brief exposure of human BM-derived HPCs to IL-1β in vitro induces a trained immunity phenotype. Competing Interests: Declaration of interests M.G.N. and L.A.B.J. are scientific founders of TTxD and Lemba TX. M.G.N. is scientific founder of Biotrip. W.H.C.R. is a consultant for Stryker for educational purposes only. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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