Simultaneous enantioseparation of citrus flavonoids in chiral microemulsion electrokinetic chromatography using deep eutectic solvents.

Autor: Jin HF; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China., Shen QX; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China., Ma XR; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China., Cao J; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China. Electronic address: caoj@hznu.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2025 Feb 01; Vol. 464 (Pt 3), pp. 141805. Date of Electronic Publication: 2024 Nov 04.
DOI: 10.1016/j.foodchem.2024.141805
Abstrakt: A novel, deep eutectic solvent (DES)-assisted sodium cholate (SC)-chiral microemulsion electrokinetic chromatography method is presented for separation of five flavonoid glycoside enantiomers, namely hesperidin, naringin, narirutin, eriocitrin, and neoeriocitrin. Herein, we develop a novel, green DES, whose addition considerably enhances the separation performance through multiple synergistic effects. A series of factors notably affecting chiral separation are systematically optimized: type and concentration of cyclodextrin, DES, and SC, oil phase type, and pH. The microemulsion comprises 0.5 % (v/v) n-octanol, 0.6 % (v/v) DES prepared using betaine and citric acid, 20 mM 2-HP-β-CD, 100 mM scdium cholate hydrate, and 15 mM borax buffer under the optimized conditions. Enantioseparations of the five enantiomers are achieved within 7 min, with resolutions ranging from 1.29 to 2.88. The method developed provides an innovative and effective way for chiral separation of enantiomers in complex food matrices.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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