Deciphering the impact and mechanism of total flavonoids from Cortex Juglandis Mandshuricae on alcoholic fatty liver employing LC-MS/MS, network pharmacology analysis and in vitro validation.

Autor: Niu T; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Wang J; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Xun L; School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Zheng B; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Deng Z; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Chen Z; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Jia K; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China., Zhao P; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China. Electronic address: 60030080@sdutcm.edu.cn., Zhao Q; School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China. Electronic address: qitaozhao@163.com.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2024 Nov 01; Vol. 1248, pp. 124334. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1016/j.jchromb.2024.124334
Abstrakt: The Cortex Juglandis Mandshuricae (CJM) has the efficacy of penetrating the liver meridian, removing heat and dampness, and alleviating the liver, which corresponds to the pathogenesis of alcoholic fatty liver disease (AFLD) with damp heat accumulation. Modern research has shown that total flavonoids from Cortex Juglandis Mandshuricae (TFC) have hepatoprotective, antioxidant and antitumour pharmacological effects. However, there is no any investigation on the mechanism of TFC improving AFLD. In this work, a valid strategy combining UPLC-Q-Exactive Orbitrap-MS, network pharmacology and in vitro cellular experimental validation is proposed to predict the targets and pathways of TFC to ameliorate AFLD and to explore its mechanism of action. As a result, 26 flavonoids and 182 targets linked to TFC and AFLD were identified. These compounds realize their critical targets via various signaling pathways and perform multiple biological functions on the basis of the constructed compound-disease target networks. In vitro experiments demonstrated TFC had a protective impact on ethanol-treated L02 cells to a certain extent and could diminished lipid accretion. In addition, RT-qPCR and western blot results illustrated that TFC could regulate the expression of PPARα, CPT-1, SREBP-1c and FAS, and inhibit alcohol-induced lipid accumulation in L02 cells thereby alleviating AFLD. The present study further provides experimental justification for TFC to ameliorate AFLD in practical applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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