| Autor: |
Liu XX; Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China., Dou H; Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China., Liu L; Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China., Wang GQ; Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China., Xiong ZQ; Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China., Ai LZ; Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. |
| Abstrakt: |
Conjugated linoleic acid (CLA) possesses anticancer, anti-inflammatory, and antiobesity properties, making it a significant research focus. In this study, we identified TetR, a TetR/AcrR family transcriptional regulator encoded by AR1031 , as the transcriptional regulator of CLA synthesis in Lactiplantibacillus plantarum AR195. TetR binds to the promoter regions of the CLA synthesis genes, including the cla operon and cla-hy , thereby enhancing CLA biosynthesis. Knockout of tetR led to the downregulation of cla-hy by 68%, cla-dh by 86%, and cla-dc by 33%, resulting in reduced CLA yield. Further bioinformatic analysis and segmented electrophoretic mobility shift assay (EMSA) experiments revealed that TetR recognizes a conserved sequence (5'-TGTAGATTG-n 4 -CTTCA-3') and binds to multiple sites within the regulatory region of the cla operon and cla-hy , thus promoting CLA biosynthesis. Overexpression of tetR increased the CLA yield by 7%. These findings provide precise insights into the regulation of CLA biosynthesis and suggest strategies for enhancing CLA production. |
