Highly specific detection of ROR1 cancer biomarker with bipolar electrochemiluminescence.

Autor: Mortazavi SMR; Nanobiosensors Lab, Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran., Hosseini M; Nanobiosensors Lab, Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran. hosseini_m@ut.ac.ir., Xu G; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China., Naderi-Manesh H; Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran., Ganjali MR; Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Nov 08; Vol. 191 (12), pp. 734. Date of Electronic Publication: 2024 Nov 08.
DOI: 10.1007/s00604-024-06815-1
Abstrakt: An electrochemiluminescence (ECL) detection system is presented integrated with a bipolar electrode system for sensitive cancer diagnosis. In order to achieve the highest electrical conductivity and redox-active surface area, MXene was chosen as the material for the bipolar electrode. As part of the detection process, the anodic pole of the bipolar electrode was modified with the receptor tyrosine kinase like orphan receptor 1 (ROR1) antibody, followed by an immunoassay using the ROR1 antibody-modified Ag triangle that was identified as significantly enhancing ECL. We measured the ECL of luminol using the anode pole of BPE as an analytical signal in the presence of H 2 O 2 . Additionally, 3D-printed microchannels were used to fabricate the BPE system, to reduce the quantity of sample required. It has been shown that the present immunosensors are low-cost and sensitive in detecting types of cancer, with an extended linear range of 10 fg mL -1 to 1 µg mL -1 in the analysis of synthetic samples and achieving an accuracy of ~ 90% in diagnosing ten unknown real samples.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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