Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion.

Autor: Di Franco C; Institituto di Fotonica e Nanotecnologia (IFN) , Consiglio Nazionale delle Ricerche (CNR), CNR IFN, Bari, 70126, Italy., Macchia E; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Bari, 70125, Italy.; Centre for Colloid and Surface Science at Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy., Catacchio M; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Bari, 70125, Italy., Caputo M; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Bari, 70125, Italy., Scandurra C; Dipartimento di Chimica and Centre for Colloid and Surface Science, Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy., Sarcina L; Dipartimento di Chimica and Centre for Colloid and Surface Science, Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy., Bollella P; Dipartimento di Chimica and Centre for Colloid and Surface Science, Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy., Tricase A; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Bari, 70125, Italy.; Centre for Colloid and Surface Science at Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy., Innocenti M; Dipartimento di Chimica, Università degli Studi di Firenze, INSTM Consortium ℅ Dip. Chimica, Via della Lastruccia 3-13, Sesto Fiorentino, I-50019, Florence, Italy., Funari R; Dipartimento Interateneo di Fisica, Università degli Studi di Bari Aldo Moro, Bari, 70125, Italy.; Istituto di Intelligenza Meccanica, Scuola Superiore Sant'Anna, Via G. Moruzzi, 1, Pisa, 56124, Italy., Piscitelli M; Institituto di Fotonica e Nanotecnologia (IFN) , Consiglio Nazionale delle Ricerche (CNR), CNR IFN, Bari, 70126, Italy.; Dipartimento Interateneo di Fisica, Università degli Studi di Bari Aldo Moro, Bari, 70125, Italy., Scamarcio G; Dipartimento Interateneo di Fisica, Università degli Studi di Bari Aldo Moro, Bari, 70125, Italy.; CNR- Istituto Nanoscienze c/o Scuola Normale Superiore, Pisa, 56127, Italy., Torsi L; Dipartimento di Chimica and Centre for Colloid and Surface Science, Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Nov 08, pp. e2412347. Date of Electronic Publication: 2024 Nov 08.
DOI: 10.1002/advs.202412347
Abstrakt: The electric dipoles of proteins in a biolayer determine their dielectric properties through the polarization density P. Hence, its reproducibility is crucial for applications, particularly in bioelectronics. Biolayers encompassing capturing antibodies covalently bound at a biosensing interface are generally preferred for their assumed higher stability. However, surface physisorption is shown to offer advantages like easily scalable fabrication processes and high stability. The present study investigates the effects of electric-field (EF)-cycling of anti-Immunoglobulin M (anti-IgM) biolayers physisorbed on Au. The impact of EF-cycling on the dielectric, optical, and mechanical properties of anti-IgM biolayer is investigated. A reduction of the dispersion (standard deviation over a set of 31 samples) of the measured P values is observed, while the set median stays almost constant. Hence, physisorption combined with EF cycling, results in a biolayer with highly reproducible bioelectronic properties. Additionally, the study provides important insights into the mechanisms of dielectric rearrangement of dipole moments in capturing biolayers after EF-cycling. Notably, EF-cycling acts as an annealing process, driving the proteins in the biolayer into a statistically more probable and stable conformational state. Understanding these phenomena enhances the knowledge of the properties of physisorbed biolayers and can inform design strategies for bioelectronic devices.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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