LPIN2 is the phosphatase dominating the penultimate step of neutral lipid biosynthesis in Dictyostelium .
| Autor: | Kappelt F; University of Kassel, Kassel, Hesse, Germany., Maniak M; University of Kassel, Kassel, Hesse, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2024 Oct 23; Vol. 2024. Date of Electronic Publication: 2024 Oct 23 (Print Publication: 2024). |
| DOI: | 10.17912/micropub.biology.001296 |
| Abstrakt: | Dictyostelium amoebae store surplus fatty acids from the diet in form of lipid droplets. Some of the enzymes governing neutral lipid synthesis are already known. For the phosphatidic acid-specific phosphatases, six genes were found, one of which was automatically annotated as LPIN2. Two GFP-tagged variants of LPIN2 homogeneously distribute in the cytoplasm and no organelle association was observed. LPIN2 - mutants contain less than 17% residual amount of the major neutral lipid species, but phospholipid amounts are not obviously affected. A growth retardation on bacteria as food source may suggest that lipid droplets serve to detoxify excess free fatty acids. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.) |
| Databáze: | MEDLINE |
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