Impact of 6-OHDA injection and microtrauma in the rat substantia nigra on local brain amyloid beta protein concentrations in the affected area.

Autor:	Roldán-Kalil JA; Department of Physiology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico., Vendrell-Gonzalez SE; Department of Microbiology and Immunology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico., Espinosa-Ponce N; Department of Microbiology and Immunology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico., Colón-Vasques J; Department of Physiology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico., Ortiz-Rivera J; Department of Physiology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico., Tsytsarev V; Department of Neurobiology, University of Maryland School of Medicine, Baltimore, MD, USA., Alves JM; Department of Microbiology and Immunology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico., Inyushin M; Department of Physiology, Universidad Central del Caribe School of Medicine, Bayamón, Puerto Rico. mikhail.inyushin@uccaribe.edu.
Jazyk:	angličtina
Zdroj:	Histology and histopathology [Histol Histopathol] 2024 Oct 22, pp. 18836. Date of Electronic Publication: 2024 Oct 22.
DOI:	10.14670/HH-18-836
Abstrakt:	Amyloid beta peptides (Aβ) are key indicators of Alzheimer's disease and are also linked to cognitive decline in Parkinson's disease (PD) and other neurodegenerative disorders. This study explored the accumulation of Aβ in a standard 6-Hydroxydopamine (6-OHDA) model of PD. We unilaterally injected 6-OHDA into the substantia nigra of Wistar rats to induce dopaminergic cell degeneration and death, a characteristic of PD. The goal was to detect Aβ protein in tissues and blood vessels showing inflammation or degeneration from the 6-OHDA injection. Our results showed that 6-OHDA injection produced a statistically significant rise in Aβ concentration at the injection site 60 minutes after injection, which was slightly reduced 24 hours post-injection but still significantly higher than in controls. We also tried Gp120 injection in the same zone but it only produced effects comparable to control needle trauma. The presence of Aβ in tissues and blood vessel walls after injection was confirmed through ELISA tests and was supported by immunohistochemical staining of injection areas. We found that the increased Aβ concentration was visible in and around blood vessels and inside blood vessel walls, and also, to a lesser extent in some cells, most probably neurons, in the area. This research highlights the connection between dopaminergic cell poisoning and the accumulation of Aβ, offering insights into the progression of PD to cognitive disorders and dementia. (©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.)
Databáze:	MEDLINE
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