Isolation, identification and optimization for tyrosinase production by banana peel waste for industrial application.
| Autor: | Jabeen R; Department of Biochemistry and Biotechnology, The Women University Multan, Multan, Pakistan., Habiba U; Department of Eastern Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan., Mustafa T; Department of Biochemistry and Biotechnology, The Women University Multan, Multan, Pakistan., Rehman T; Department of BHMS, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan. |
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| Jazyk: | angličtina |
| Zdroj: | Natural product research [Nat Prod Res] 2024 Nov 08, pp. 1-5. Date of Electronic Publication: 2024 Nov 08. |
| DOI: | 10.1080/14786419.2024.2426209 |
| Abstrakt: | The study aimed to isolate, purify and optimise tyrosinase from banana peel waste for industrial use. Tyrosinase was extracted from banana peel using phosphate buffer. Purification initiated with centrifugation followed by ammonium sulphate precipitation. Further purification and characterisation was done through gel filtration. Bradford assay was used to determine the protein concentration. Enzyme activity of each sample was measured using tyrosinase activity assay. The pH and temperature optimisation of tyrosinase were also obtained. Results indicated that fractions obtained through ammonium sulphate precipitation at 70% saturation showed more activity than that of crude extract and 35% saturated fractions. Gel filtration column results showed that fraction number 17 &18 have maximum activity. Tyrosinase showed maximum activity at pH 7.0; 37 °C. Comparison between industrially purified and lab-isolated enzyme showed that both have similar optimum pH and temperature. It can be concluded that banana peel can be a source for synthesis of tyrosinase. |
| Databáze: | MEDLINE |
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